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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제11호
- 발행연도
- 2015.1
- 수록면
- 1,842 - 1,848 (7page)
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Cyanidin-3-glucoside (C3G) has been known to be more bioavailable than cyanidin-3- rutinoside (C3R), the most abundant anthocyanin in black raspberry (Rubus occidentalis). The aim of this study was to enhance the bioavailability of anthocyanins in black raspberry by cleaving L-rhamnose in C3R using crude enzyme extracts (CEEs) from Aspergillus usamii KCTC 6956, A. awamori KCTC 60380, A. niger KCCM 11724, A. oryzae KCCM 12698, and A. kawachii KCCM 32819. The enzyme activities of the CEEs were determined by a spectrophotometric method using ρ-nitrophenyl-rhamnopyranoside and ρ-nitrophenyl-glucopyranoside. The CEE from A. usamii had the highest α-L-rhamnosidase activity with 2.73 U/ml at 60oC, followed by those from A. awamori and A. niger. When bioconversion of C3R to C3G in black raspberry was analyzed by HPLC-DAD, the CEEs from A. usamii and A. awamori hydrolyzed 95.7% and 95.6% of C3R to C3G, respectively, after 2 h incubation. The CEEs from A. kawachii and A. oryzae did not convert C3R to C3G in black raspberry.
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참고문헌 (24)
참고문헌 신청
Abe K / 2006 / Impact of Aspergillus oryzae genomics on industrial production of metabolites / Mycopathologia 162 : 143 ~ 153
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72 :
Bram B / 1965 / Production of the enzyme naringinase by Aspergillus niger / Appl. Microbiol 13 : 842 ~ 845
Fuse M / 1988 / Process for preparing L-rhamnose / US Patent No. 4,758,283 : 283 ~
Gallego M / 2001 / Purification and characterization of an α-L-rhamnosidase from Aspergillus terreus of interest in winemaking / J. Food Sci 66 : 204 ~ 209
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72 :
Bram B / 1965 / Production of the enzyme naringinase by Aspergillus niger / Appl. Microbiol 13 : 842 ~ 845
Fuse M / 1988 / Process for preparing L-rhamnose / US Patent No. 4,758,283 : 283 ~
Gallego M / 2001 / Purification and characterization of an α-L-rhamnosidase from Aspergillus terreus of interest in winemaking / J. Food Sci 66 : 204 ~ 209
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