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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제4호
- 발행연도
- 2018.1
- 수록면
- 588 - 596 (9page)
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An endo-β-1,4-glucanase gene, cel9K, was cloned using the shot-gun method from Paenibacillus sp. X4, which was isolated from alpine soil. The gene was 2,994 bp in length, encoding a protein of 997 amino acid residues with a predicted signal peptide composed of 32 amino acid residues. Cel9K was a multimodular enzyme, and the molecular mass and theoretical pI of the mature Cel9K were 103.5 kDa and 4.81, respectively. Cel9K contains the GGxxDAGD, PHHR, GAxxGG, YxDDI, and EVxxDYN motifs found in most glycoside hydrolase family 9 (GH9) members. The protein sequence showed the highest similarity (88%) with the cellulase of Bacillus sp. BP23 in comparison with the enzymes with reported properties. The enzyme was purified by chromatography using HiTrap Q, CHT-II, and HiTrap Butyl HP. Using SDSPAGE/ activity staining, the molecular mass of Cel9K was estimated to be 93 kDa, which is a truncated form produced by the proteolytic cleavage of its C-terminus. Cel9K was optimally active at pH 5.5 and 50°C and showed a half-life of 59.2 min at 50°C. The CMCase activity was increased to more than 150% in the presence of 2 mM Na+, K+, and Ba2+, but decreased significantly to less than 50% by Mn2+ and Co2+. The addition of Cel9K to a commercial enzyme set (Celluclast 1.5L + Novozym 188) increased the saccharification of the pretreated reed and rice straw powders by 30.4% and 15.9%, respectively. The results suggest that Cel9K can be used to enhance the enzymatic conversion of lignocellulosic biomass to reducing sugars as an additive.
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참고문헌 (40)
참고문헌 신청
Balat M / 2009 / Recent trends in global production and utilization of bioethanol fuel / Appl. Energy 86:2273~2282
Saini JK / 2015 / Lignocellulosic agriculture wastes as biomass feedstocks for second-generation bioethanol production: concepts and recent developments / 3 Biotech. 5:337~353
Lynd LR / 2017 / Cellulosic ethanol: status and innovation / Curr. Opin. Biotechnol. 45:202~211
Kuhad RC / 2011 / Microbial cellulases and their industrial applications / Enzyme Res. 2011
Kasana RC / 2011 / Cellulases from psychrophilic microorganisms: a review / J. Basic Microbiol. 51:572~579
Saini JK / 2015 / Lignocellulosic agriculture wastes as biomass feedstocks for second-generation bioethanol production: concepts and recent developments / 3 Biotech. 5:337~353
Lynd LR / 2017 / Cellulosic ethanol: status and innovation / Curr. Opin. Biotechnol. 45:202~211
Kuhad RC / 2011 / Microbial cellulases and their industrial applications / Enzyme Res. 2011
Kasana RC / 2011 / Cellulases from psychrophilic microorganisms: a review / J. Basic Microbiol. 51:572~579
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