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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제7호
- 발행연도
- 2018.1
- 수록면
- 1,078 - 1,085 (8page)
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초록· 키워드
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A salt-tolerant cellulase secreted by a marine Bacillus sp. SR22 strain with wide resistance to temperature and pH was purified and characterized. Its approximate mass was 37 kDa. The endoglucanase, named as Bc22Cel, was purified by ammonium sulfate precipitation, gel filtration chromatography, and extraction from the gel after non-reducing sodium dodecyl sufate-polyacrylamide gel electrophoresis. The optimal pH value and temperature of Bc22Cel were 6.5 and 60°C, respectively. The purified Bc22Cel showed a considerable halophilic property, being able to maintain more than 70% of residual activity even when pre-incubated with 1.5 M NaCl for 1 h. Kinetic analysis of the purified enzyme showed the K m and Vmax to be 0.704 mg/ml and 29.85 μmol·ml-1·min-1, respectively. Taken together, the present data indicate Bc22Cel as a potential and useful candidate for industrial applications, such as the bioconversion of sugarcane bagasse to its derivatives.
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참고문헌 (31)
참고문헌 신청
Ragauskas AJ / 2006 / The path forward for biofuels and biomaterials / Science 311:484~489
Jia L / 2015 / Effect of pretreatment methods on the synergism of cellulase and xylanase during the hydrolysis of bagasse / Bioresour. Technol. 185
Dantur KI / 2015 / Isolation of cellulolytic bacteria from the intestine of Diatraea saccharalis larvae and evaluation of their capacity to degrade sugarcane biomass / AMB Exp. 5:15
Yin L-J / 2010 / Purification and characterization of a cellulase from Bacillus subtilis / J. Mar. Sci. Technol. 18:466~471
Lynd LR / 2002 / Microbial cellulose utilization: fundamentals and biotechnology / Bioresour. Technol. 66:506~577
Jia L / 2015 / Effect of pretreatment methods on the synergism of cellulase and xylanase during the hydrolysis of bagasse / Bioresour. Technol. 185
Dantur KI / 2015 / Isolation of cellulolytic bacteria from the intestine of Diatraea saccharalis larvae and evaluation of their capacity to degrade sugarcane biomass / AMB Exp. 5:15
Yin L-J / 2010 / Purification and characterization of a cellulase from Bacillus subtilis / J. Mar. Sci. Technol. 18:466~471
Lynd LR / 2002 / Microbial cellulose utilization: fundamentals and biotechnology / Bioresour. Technol. 66:506~577
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