지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제31권 제10호
- 발행연도
- 2021.10
- 수록면
- 1,446 - 1,454 (9page)
- DOI
- 10.4014/jmb.2105.05026
이용수
초록· 키워드
오류제보하기
Herein, we cloned and expressed an endo-β-1,4-glucanase gene (celA1805) from Bacillus subtilis B111 in Escherichia coli. The recombinant celA1805 contains a glycosyl hydrolase (GH) family 8 domain and shared 76.8% identity with endo-1,4-β-glucanase from Bacillus sp. KSM-330. Results showed that the optimal pH and temperature of celA1805 were 6.0 and 50oC, respectively, and it was stable at pH 3-9 and temperature ≤50°C. Metal ions slightly affected enzyme activity, but chemical agents generally inhibited enzyme activity. Moreover, celA1805 showed a wide substrate specificity to CMC, barley β-glucan, lichenin, chitosan, PASC and avicel. The Km and Vmax values of celA1805 were 1.78 mg/ml and 50.09 μmol/min/mg. When incubated with cellooligosaccharides ranging from cellotriose to cellopentose, celA1805 mainly hydrolyzed cellotetrose (G4) and cellopentose (G5) to cellose (G2) and cellotriose (G3), but hardly hydrolyzed cellotriose. The concentrations of reducing sugars saccharified by celA1805 from wheat straw, rape straw, rice straw, peanut straw, and corn straw were increased by 0.21, 0.51, 0.26, 0.36, and 0.66 mg/ml, respectively. The results obtained in this study suggest potential applications of celA1805 in biomass saccharification.
목차
등록된 정보가 없습니다.
참고문헌 (45)
참고문헌 신청
Handelsman J / 1998 / Molecular biological access to the chemistry of unknown soil microbes : a new frontier for natural products / Chem. Biol 5:R245~249
Haghighi Mood S / 2013 / Lignocellulosic biomass to bioethanol, a comprehensive review with a focus on pretreatment / Renew. Sustain. Energy Rev 27:77~93
Judyta Strakowska / 2014 / The significance of cellulolytic enzymes produced by Trichoderma in opportunistic lifestyle of this fungus / J. Basic Microbiol 54(Suppl 1):S2~13
Liu D / 2011 / Expression, purification and characterization of two thermostable endoglucanases cloned from a lignocellulosic decomposing fungi Aspergillus fumigatus Z5 isolated from compost / Protein Expr. Purif 79:176~186
Yan J / 2016 / Functional and structural analysis of Pichia pastoris-expressed Aspergillus niger 1, 4-β-endoglucanase / Biochem. Biophys. Res. Commun 475:8~12
Haghighi Mood S / 2013 / Lignocellulosic biomass to bioethanol, a comprehensive review with a focus on pretreatment / Renew. Sustain. Energy Rev 27:77~93
Judyta Strakowska / 2014 / The significance of cellulolytic enzymes produced by Trichoderma in opportunistic lifestyle of this fungus / J. Basic Microbiol 54(Suppl 1):S2~13
Liu D / 2011 / Expression, purification and characterization of two thermostable endoglucanases cloned from a lignocellulosic decomposing fungi Aspergillus fumigatus Z5 isolated from compost / Protein Expr. Purif 79:176~186
Yan J / 2016 / Functional and structural analysis of Pichia pastoris-expressed Aspergillus niger 1, 4-β-endoglucanase / Biochem. Biophys. Res. Commun 475:8~12
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Characterization of a Multimodular Endo-β-1,4-Glucanase (Cel9K) from Paenibacillus sp. X4 with a Potential Additive for Saccharification
Journal of Microbiology and Biotechnology
2018 .01
-
Thermal Stability and Soft Magnetic Properties of Novel Low-Coercivity Fe-B-Ce-La Alloys
Journal of Magnetics
2024 .09
-
돼지 정자 동결보존에 있어 5 ml straw의 한계성 극복
생명과학회지
2020 .01
-
Complete genome sequence of Bacillus subtilis BS16045 isolated from Gochujang
미생물학회지
2017 .03
-
Description of Novel Strain Bizionia sp. 2012CJ3-1 Isolated from Marine Sponge Cliona celata Grant
한국미생물학회 학술대회논문집
2022 .10
-
Roles of Carbohydrate-Binding Module (CBM) of an Endo-β-1,4- Glucanase (Cel5L) from Bacillus sp. KD1014 in Thermostability and Small-Substrate Hydrolyzing Activity
Journal of Microbiology and Biotechnology
2018 .01
-
Enzymatic Characterization of γ-Glutamyl Hydrolase from Bacillus licheniformis DSM 13 Heterologously Expressed in Bacillus subtilis
한국미생물학회 학술대회논문집
2020 .10
-
Complete genome sequence of Bacillus subtilis PN176 with high fibrinolytic enzyme activity isolated from Cheonggukjang
미생물학회지
2022 .12
-
김치로부터 단백질 분해 효소활성이 우수한 Bacillus subtilis 균주의 분리
한국미생물·생명공학회지
2017 .01
-
Structural Study on a Butanol Dehydrogenase from Bacillus subtilis
한국미생물학회 학술대회논문집
2017 .04
-
Discrimination of Bacillus subtilis from other Bacillus Species Using Specific Oligonucleotide Primers for the Pyruvate Carboxylase and Shikimate Dehydrogenase Genes
Journal of Microbiology and Biotechnology
2022 .08
-
Fibrin 분해 효소-생성 Bacillus subtilis subsp. subtilis BYSnat3의 분리 및 특성
미생물학회지
2019 .12
-
Bacillus subtilis DS660과 Paenibacillus polymyxa DS842의 항균활성
미생물학회지
2018 .12
-
Properties of Antimicrobial Substances Produced by Bacillus species Isolated from Rice Straw
한국미생물·생명공학회지
2017 .06
-
된장으로부터 분리한 Bacillus subtilis CK-2가 생산하는 가수분해효소의 활성 특성
생명과학회지
2017 .07
-
Characterization of a Thermostable Lichenase from Bacillus subtilis B110 and Its Effects on β-Glucan Hydrolysis
Journal of Microbiology and Biotechnology
2022 .04
-
강원도 평창군 백룡동굴에서 산출되는 종유관의 기재학적 연구
한국지구과학회지
2017 .01
-
Isolation of Bacillus subtilis SCM146 Having Antimicrobial Activity and Medium Optimization for Improving Biomass by Response Surface Methodology
한국미생물학회 학술대회논문집
2016 .04
-
Identification and Characterization of a Bacteriocin from the Newly Isolated Bacillus subtilis HD15 with Inhibitory Effects against Bacillus cereus
Journal of Microbiology and Biotechnology
2022 .11
-
The Endo-β-1,4-Glucanase of Bacillus amyloliquefaciens Is Required for Optimum Endophytic Colonization of Plants
Journal of Microbiology and Biotechnology
2016 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0