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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제12호
- 발행연도
- 2018.1
- 수록면
- 2,036 - 2,045 (10page)
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An endo-β-1,4-glucanase gene, cel5L, was cloned using the shot-gun method from Bacillus sp.. The gene, which contained a predicted signal peptide, encoded a protein of 496 amino acid residues, and the molecular mass of the mature Cel5L was estimated to be 51.8 kDa. Cel5L contained a catalytic domain of glycoside hydrolase (GH) family 5 and a carbohydrate-binding module family 3 (CBM_3). Chromatography using HiTrap Q and CHT-II resulted in the isolation of two truncated forms corresponding to 50 (Cel5L-p50) and 35 kDa (Cel5L-p35, CBM_3-deleted form). Both enzymes were optimally active at pH 4.5 and 55°C, but had different half-lives of 4.0 and 22.8 min, respectively, at 70°C. The relative activities of Cel5Lp50 and Cel5L-p35 for barley β-glucan were 377.0 and 246.7%, respectively, compared to those for carboxymethyl-cellulose. The affinity and hydrolysis rate of pNPC by Cel5L-p35 were 1.7 and 3.3 times higher, respectively, than those by Cel5L-p50. Additions of each to a commercial enzyme set increased saccharification of pretreated rice straw powder by 17.5 and 21.0%, respectively. These results suggest CBM_3 is significantly contributing to thermostability, and to affinity and substrate specificity for small substrates, and that these two enzymes could be used as additives to enhance enzymatic saccharification.
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참고문헌 (9)
참고문헌 신청
Lynd LR / 2017 / Cellulosic ethanol: status and innovation / Curr. Opin. Biotechnol 45:202211
Zheng F / 2018 / Enhancing the catalytic activity of a novel GH5 cellulase GtCel5 from Gloeophyllum trabeum CBS 900.73 by sitedirected mutagenesis on loop 6 / Biotechnol. Biofuels 11:76
Lima AO / 2005 / Molecular characterization of a β-1,4-endoglucanase from an endophytic Bacillus pumilus strain / Appl. Microbiol. Biotechnol 68:5765
Berlemont R / 2016 / Glycoside hydrolases across environmental microbial communities / PLoS Comput. Biol 12:e1005300
Berlemont R / 2013 / Phylogenetic distribution of potential cellulases in bacteria / Appl. Environ. Microbiol 79:1545~1554
Zheng F / 2018 / Enhancing the catalytic activity of a novel GH5 cellulase GtCel5 from Gloeophyllum trabeum CBS 900.73 by sitedirected mutagenesis on loop 6 / Biotechnol. Biofuels 11:76
Lima AO / 2005 / Molecular characterization of a β-1,4-endoglucanase from an endophytic Bacillus pumilus strain / Appl. Microbiol. Biotechnol 68:5765
Berlemont R / 2016 / Glycoside hydrolases across environmental microbial communities / PLoS Comput. Biol 12:e1005300
Berlemont R / 2013 / Phylogenetic distribution of potential cellulases in bacteria / Appl. Environ. Microbiol 79:1545~1554
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