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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제17권 제5호
- 발행연도
- 2007.1
- 수록면
- 712 - 720 (9page)
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초록· 키워드
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Chinese hamster ovary (rCHO) clones in response to culturepH and temperature, serum-free suspension cultures of twoantibody-producing CHO clones (clones A and B), which wereisolated from the same parental clone by the limiting dilutionmethod, were performed in a bioreactor at pH values in therange of 6.8-7.6, and two diferent temperatures, 33oC and37oC. In regard to cell growth, clone A and clone B displayedresponse difered. In contrast, clones A and B displayed diferentresponses to temperature in regard to antibody production. Inthe case of clone A, no significant increase in maximumantibody concentration was achieved by lowering the culturetemperature. The maximum antibody concentration obtainedat 33oC (pH 7.4) and 37oC (pH 7.0) were 82.0±2.6 and73.2±4.1g/ml, respectively. On the other hand, in the caseof clone B, an aproximately 2.5-fold increase in maximumantibody concentration was achieved by lowering the culturetemperature. The enhanced maximum antibody concentrationof clone B at 3oC (132.6±14.9g/ml at pH 7.2) was due tonot only enhanced specific antibody productivity but also toprolonged culture longevity. At 33oC, the culture longevity ofTaken together, CHO clones derived from the same parentalclone displayed quite different responses to culture temperatureand pH with regards antibody production, sugesting thatenvironmental parameters such as temperature and pH shouldbe optimized for each CHO clone.
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참고문헌 (22)
참고문헌 신청
Borys / 1993 / Culture pH affects expression rates and glycosylation of recombinant mouse placental lactogen proteins by Chinese hamster ovary : 720 ~ 724
Clark, K. J. R / 2004 / Temperature effects on product-quality-related enzymes in batch CHO cell cultures producing recombinant tPA 20 : 1888 ~ 1892
Davies, J / 2001 / Chromosome localization and gene-copy-number quantification of three random integrations in Chinese hamster ovary cells and their amplified cell lines usin
Flintoff / 1984 / Moderate-level gene amplification in methotrexateresistant Chinese hamster ovary cells is accompanied by chromosomal translocations at or near the site of t
Fogolin, M. B / 2005 / Temperature reduction in cultures of hGM-CSF-expressing CHO cells: Effect on productivity and product quality 21 : 17 ~ 21
Clark, K. J. R / 2004 / Temperature effects on product-quality-related enzymes in batch CHO cell cultures producing recombinant tPA 20 : 1888 ~ 1892
Davies, J / 2001 / Chromosome localization and gene-copy-number quantification of three random integrations in Chinese hamster ovary cells and their amplified cell lines usin
Flintoff / 1984 / Moderate-level gene amplification in methotrexateresistant Chinese hamster ovary cells is accompanied by chromosomal translocations at or near the site of t
Fogolin, M. B / 2005 / Temperature reduction in cultures of hGM-CSF-expressing CHO cells: Effect on productivity and product quality 21 : 17 ~ 21
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