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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제11호
- 발행연도
- 2010.1
- 수록면
- 1,481 - 1,483 (3page)
이용수
초록· 키워드
오류제보하기
We constructed an efficient T-vector, pTQEST216T that employed an engineered esterase as an indicator for direct cloning of PCR products. After ligation of the XcmIdigested vector with PCR products, this cloning system could easily discriminate positive clones owing to insertional inactivation of the esterase reporter. Additionally, PCR products were efficiently cloned into this vector without the gel purification steps, owing to the well-designed multi-cloning site that was in-frame fused at the circularly permutated gap of the reporter.
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등록된 정보가 없습니다.
참고문헌 (11)
참고문헌 신청
Agafonov, D. E / 2005 / The esterase from Alicyclobacillus acidocaldarius as a reporter enzyme and affinity tag for protein biosynthesis / FEBS Lett 579 : 2082 ~ 2086
Cheong, D. E / 2009 / A new cloning system using a mutant esterase containing MCS as an indicator for gene cloning / J. Microbiol. Methods 77 : 302 ~ 307
Cuff, J. A / 2000 / Application of multiple sequence alignment profiles to improve protein secondary structure prediction / Protein Struct. Funct. Genet 40 : 502 ~ 511
Holton, T. A / 1991 / A simple and efficient method for direct cloning of PCR products using ddT-tailed vectors / Nucleic Acids Res 19 : 1156 ~
Inouye, S / 1997 / A bacterial cloning vector using a mutated Aequorea green fluorescent protein as an indicator / Gene 189 : 159 ~ 162
Cheong, D. E / 2009 / A new cloning system using a mutant esterase containing MCS as an indicator for gene cloning / J. Microbiol. Methods 77 : 302 ~ 307
Cuff, J. A / 2000 / Application of multiple sequence alignment profiles to improve protein secondary structure prediction / Protein Struct. Funct. Genet 40 : 502 ~ 511
Holton, T. A / 1991 / A simple and efficient method for direct cloning of PCR products using ddT-tailed vectors / Nucleic Acids Res 19 : 1156 ~
Inouye, S / 1997 / A bacterial cloning vector using a mutated Aequorea green fluorescent protein as an indicator / Gene 189 : 159 ~ 162
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