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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제16권 제2호
- 발행연도
- 2006.1
- 수록면
- 256 - 263 (8page)
이용수
초록· 키워드
오류제보하기
In the model legume Medicago truncatula, twosickle and sunn, exhibit morphologically and geneticallydistinct hypernodulation phenotypes. However, efforts to isolatethe single recessive and single semidominant genes for sickleand sunn, respectively, by map-based cloning have so farbeen unsuccessful, partly due to the absence of clones thatenable walks from linked marker positions. To help resolvethese difficulties, a new bacterial artificial chromosome (BAC)library was constructed using BamHI-digested genomicfragments. A total of 23,808 clones were colected fromligation mixtures prepared with double-size-selected high-molecular-weight DNA. The average insert size was 116 kbbased on an analysis of 88 randomly selected clones usingNotI digestion and pulsed-field gel electrophoresis. About18.5% of the library clones lacked inserts. The frequency ofthe BAC clones carrying chloroplast or mitochondrial DNAwas 0.98% and 0.03%, respectively. The library representedaproximately 4.9 haploid M. truncatulaof the BAC clone filters with a C0t-1 DNA probe revealedthat approximately 37% of the clones likely carried repetitivesequence-enriched DNA. An ordered array of pooled BACDNA was screened by polymerase chain reactions using 13sequence-characterized molecular markers that belonged tothe eight linkage groups. Except for two markers, one to fivepositive BAC clones were obtained per marker. Accordingly,the sickle- and sunnbe useful for the isolation of these biotechnologically importantgenes. The new library will also provide clones that fill thegaps between preexisting BAC contigs, facilitating the physicalmaping and genome sequencing of M. truncatula.
목차
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참고문헌 (23)
참고문헌 신청
Albrecht, C / 1999 / Legume nodulation and mycorrhizae formation; Two extremes in host specificity meet / EMBO J. 18 : 281 ~ 288
Ane, J.-M / 2004 / Medicago truncatula DMI1 required for bacterial and fungal symbioses in legumes / Science 303 : 1364 ~ 1367
BAEK, CHANG-HO / 2004 / Genes for the Catabolism of Deoxyfructosyl Glutamine in pAtC58 Are Attributed to Utilization of Octopine in Agrobacterium tumefaciens Strain NT1 / Jou
Barker, D. G / 1990 / Medicago truncatula, a model plant for studying the molecular genetics of the Sinorhizobium-legume symbiosis / Plant Mol. Biol. Rep. 8 : 40 ~ 49
Blondon, F / 1994 / Genome size and base composition in Medicago sativa and M. truncatula species / Genome 37 : 264 ~ 270
Ane, J.-M / 2004 / Medicago truncatula DMI1 required for bacterial and fungal symbioses in legumes / Science 303 : 1364 ~ 1367
BAEK, CHANG-HO / 2004 / Genes for the Catabolism of Deoxyfructosyl Glutamine in pAtC58 Are Attributed to Utilization of Octopine in Agrobacterium tumefaciens Strain NT1 / Jou
Barker, D. G / 1990 / Medicago truncatula, a model plant for studying the molecular genetics of the Sinorhizobium-legume symbiosis / Plant Mol. Biol. Rep. 8 : 40 ~ 49
Blondon, F / 1994 / Genome size and base composition in Medicago sativa and M. truncatula species / Genome 37 : 264 ~ 270
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