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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제1호
- 발행연도
- 2019.1
- 수록면
- 55 - 58 (4page)
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Development of stable rCHO cell lines is still time consuming and labor intensive, although it is a critical step in the commercial development of recombinant antibodies. The current work demonstrates, for the first time, that electroporation of CHO cells with DMSO can enhance stable expression of recombinant antibodies in rCHO cells. Electroporation with DMSO resulted in an average 3.7-fold and 2.8-fold increases in expression levels of aflibercept and pembrolizumab, respectively, in pools of stable rCHO cells. It also resulted in an average of 2.2-fold and 2.6-fold increases in the expression of aflibercept and pembrolizumab, respectively, in single-cell derived rCHO clones. Simple batch cultures of rCHO cell clones with the highest expression produced 1.0 g/l for aflibercept and 1.4 g/l for pembrolizumab without a time-consuming gene amplification process. Electroporation with DMSO also shortened the development of rCHO cell lines to 2-3 months, allowing rapid establishment of stable rCHO cell lines with a desirable expression level antibodies.
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참고문헌 (14)
참고문헌 신청
Jesus MD / 2011 / Manufacturing recombinant proteins in kg-ton quantities using animal cells in bioreactors / Eur. J. Pharm. Biopharm 78:184~188
Durocher Y / 2009 / Expression systems for therapeutic glycoprotein production / Curr. Opin. Biotechnol 20:700~707
Dietmair S / 2010 / Engineering a mammalian super producer / J. Chem. Technol. Biotechnol 86:905~914
Noh SM / 2013 / Development of recombinant Chinese hamster ovary cell lines for therapeutic protein production / Curr Opin. Chem. Eng 2:391~397
Baldi L / 2007 / Recombinant protein production by large-scale transient gene expression in mammalian cells: state of art and future perspectives / Biotechnol. Lett 29:677~684
Durocher Y / 2009 / Expression systems for therapeutic glycoprotein production / Curr. Opin. Biotechnol 20:700~707
Dietmair S / 2010 / Engineering a mammalian super producer / J. Chem. Technol. Biotechnol 86:905~914
Noh SM / 2013 / Development of recombinant Chinese hamster ovary cell lines for therapeutic protein production / Curr Opin. Chem. Eng 2:391~397
Baldi L / 2007 / Recombinant protein production by large-scale transient gene expression in mammalian cells: state of art and future perspectives / Biotechnol. Lett 29:677~684
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