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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제7호
- 발행연도
- 2008.1
- 수록면
- 1,342 - 1,351 (10page)
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The feasibility of the use of Flp-mediated cassette exchange
in the development of a CHO cell line, which produces
erythropoietin (EPO) stably and largely, was investigated.
A stable, high enhanced green fluorescence protein
(EGFP)-producing clone was screened by extensive flow
cytometric analysis. An EPO expression unit was targeted
into the premarked locus of the stable parental clone by
Flp-mediated cassette exchange and a correctly targeted
clone (FC28T7) was obtained. The EPO production of
FC28T7 was proven to be stable in long-term culture.
Furthermore, the Flp-mediated cassette exchange did not
alter the stable parental clone’s characteristics concerning
transgene expression level and stability. Taken together,
the data obtained here indicated that the establishment of
CHO cell lines stably producing a desired protein is
achievable using Flp-mediated cassette exchange.
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참고문헌 (21)
참고문헌 신청
Andreas, S. / 2002 / Enhanced efficiency through nuclear localization signal fusion on phage φC31-integrase: Activity comparison with Cre and FLPe recombinase in mammalian c
Barnes, L. M. / 2003 / Stability of protein production from recombinant mammalian cells. Biotechnol / Bioeng 81 : 631 ~ 639
Belteki, G. / 2003 / Site-specific cassette exchange and germline transmission with mouse ES cells expressing φC31 integrase. Nat / Biotechnol 21 : 321 ~ 324
Cheng, E. Y. / 2005 / A system for precise analysis of transcription-regulating elements of immunoglobulin genes / BMC Biotechnol 5 (27)
Coroadinha, A. S. / 2006 / The use of recombinase mediated cassette exchange in retroviral vector producer cell lines: Predictability and efficiency by transgene exchange / B
Barnes, L. M. / 2003 / Stability of protein production from recombinant mammalian cells. Biotechnol / Bioeng 81 : 631 ~ 639
Belteki, G. / 2003 / Site-specific cassette exchange and germline transmission with mouse ES cells expressing φC31 integrase. Nat / Biotechnol 21 : 321 ~ 324
Cheng, E. Y. / 2005 / A system for precise analysis of transcription-regulating elements of immunoglobulin genes / BMC Biotechnol 5 (27)
Coroadinha, A. S. / 2006 / The use of recombinase mediated cassette exchange in retroviral vector producer cell lines: Predictability and efficiency by transgene exchange / B
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