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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제16권 제1호
- 발행연도
- 2006.1
- 수록면
- 153 - 157 (5page)
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A soil metagenomic library was constructed using anE. coli-fosmid cloning system with environmental DNAs extractedfrom Kwangreung forest topsoil. We targeted the genes involvedin the biosynthesis of bacterial polyketides. Initially, a total of36 clone pools (10,800 clones) were explored by the PCR-basedmethod using the metagenomic DNAs from each pool and adegenerate primer set, which has been designed based on thehighly conserved regions among ketoacyl synthase (KS) domainsclone pools were tentatively selected as positive and furtherexamined through a hybridization-based method for selectinga fosmid clone containing PKS I genes. Colony hybridizationwas performed against fosmid clones from the 6 positivepools, and finaly 4 clones were picked out and confirmed tocontain the conserved DNA fragment of KS domains. In thisstudy, we present a simple and feasible sorting method for adesired clone from metagenomic libraries.
#Soil metagenome
#sequence-based screening
#polyketide synthaseSecondary metabolites produced by microbes have beenimportant sources of bioactive molecules such as antibioticsand other pharmaceutical agents over the last 60 years.However
#the discover
#Soil metagenome
#sequence-based screening
#polyketide synthaseSecondary metabolites produced by microbes have beenimportant sources of bioactive molecules such as antibioticsand other pharmaceutical agents over the last 60 years.However
#the discover
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참고문헌 (22)
참고문헌 신청
Altschul, S / 1997 / Gapped BLAST and PSIBLAST: A new generation of protein database search programs / Nucleic Acids Res. 25 : 3389 ~ 3402
Amann, R. I / 1995 / Phylogenetic identification and in situ detection of individual microbial cells without cultivation / Microbiol. Rev. 59 : 143 ~ 169
Aparicio, J. F / 2000 / A complex multienzyme system encoded by five polyketide synthase genes is involved in the biosynthesis of the 26-membered polyene macrolide pimaricin
August, P. R / 1998 / Biosynthesis of the ansamycin antibiotic rifamycin: Deductions from the molecular analysis of the rif biosynthetic gene cluster of Amycolatopsis mediter
Courtois, S / 2003 / Recombinant environmental libraries provide access to microbial diversity for drug discovery from natural products / Appl. Environ. Microbiol. 69 : 49 ~
Amann, R. I / 1995 / Phylogenetic identification and in situ detection of individual microbial cells without cultivation / Microbiol. Rev. 59 : 143 ~ 169
Aparicio, J. F / 2000 / A complex multienzyme system encoded by five polyketide synthase genes is involved in the biosynthesis of the 26-membered polyene macrolide pimaricin
August, P. R / 1998 / Biosynthesis of the ansamycin antibiotic rifamycin: Deductions from the molecular analysis of the rif biosynthetic gene cluster of Amycolatopsis mediter
Courtois, S / 2003 / Recombinant environmental libraries provide access to microbial diversity for drug discovery from natural products / Appl. Environ. Microbiol. 69 : 49 ~
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