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초록· 키워드
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To better understand gene expression at very low levels, we have designed a method to eliminate cDNA clones representing abundant mRNAs. A cDNA library for drought-stressed hot pepper (Capsicum annuum) (Choi et al., 2002) underwent double-negative screening, once with probes made from a drought-stressed plant, the second time, with probes from a non-stressed plant. The cDNA clones that showed very weak or negative signals were isolated for further analysis, which resulted in 1399 cDNA clones from about 20,000 screened clones. When nucleotide sequences were determined, we obtained 1142 tentative unique genes, with a redundancy rate of 20.41%. An homology database search for the deduced amino acid sequences revealed that about 79% of the cDNA clones could not be matched for functioning with previously characterized sequences. However, when these uncategorized clones were subjected to classification based on functional domains, most could be cited. Notably, clones with possible functions in RNA transport, protein synthesis, and regulation of protein activity showed a dramatic increase in appearance while those coding for transposable elements, viral proteins, and plasmid proteins occupied a much smaller portion compared with those in the Arabidopsis thaliana genome. In addition, those coding for proteins targeted to the endoplasmic reticulum were dramatically more abundant in our clones compared with the Arabidopsis database.
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Isolation and Characterization of Drought-Induced cDNA Clones from Hot Pepper (Capsicum annuum)
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Isolation and Characterization of Glutathione S-Transferase cDNA Clone in Capsicum annuum L.
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Structure and Expression Pattern of Antifungal Protein cDNA Clone from Capsicum annum L.
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Analysis of 176 Expressed Sequence Tags Generated from cDNA Clones of Hot Pepper by Single-pass Sequencing
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Isolation and Characterization of Acid Invertase cDNA Clone in Hot Pepper (Capsicum annuum L.) Fruits
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Molecular Characterization of a cDNA for a Cysteine-Rich Antifungal Protein from Capsicum annuum
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Effect of Water Stress on Photosynthesis in Pepper (Capsicum annuum L.)
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Differential Expression of Catalase Genes in Hot Pepper (Capsicum annuum L.)
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Chromosomal Information of 1,144 Korean BAC Clones
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Regulation of Nitrite Reductase and its Transcript Level in Hot Pepper(Capsicum annuum L.)
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