지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Bacterial infection and smoking are an important risk factors involved in the development and progression of periodontitis. However, the signalingmechanism underlying the host immune response is not fully understood in periodontal lesions. In this study, we determined the expression of januskinase (JAK)/signal transducer and activator of transcription (STAT) on Porphyromonas gingivalis lipopolysaccharide (LPS)- and nicotine-inducedcytotoxicity and the production of inflammatory mediators, using osteoblasts. The cells were cultured with 5 mM nicotine in the presence of 1 μg/mlLPS. Cell viability was determined using MTT assay. The role of JAK on inflammatory mediator expression and production, and the regulatorymechanisms involved were assessed via enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction, and Western blotanalysis. LPS- and nicotine synergistically induced the production of cyclooxgenase-2 (COX-2) and prostaglandin E2 (PGE2) and increased theprotein expression of JAK/STAT. Treatment with an JAK inhibitor blocked the production of COX-2 and PGE2 as well as the expression ofpro-inflammatory cytokines, such as tumor necrosis factor-α, interleukin-1β (IL-1β), and IL-6 in LPS- and nicotine-stimulated osteoblasts. Theseresults suggest that JAK/STAT is closely related to the LPS- and nicotine-induced inflammatory effects and is likely to regulate the immune responsein periodontal disease associated with dental plaque and smoking.
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참고문헌 (26)
참고문헌 신청
Starr R / 1999 / Negative regulation of the JAK/STAT pathway / Bioessays 21:47~52
Schindler CW / 2002 / Series introduction. JAK-STAT signaling in human disease / J Clin Invest 109:1133~1137
Alpar B / 1998 / Nicotine-induced alterations in human primary periodontal ligament and gingiva fibroblast cultures / Clin Oral Investig 2 : 40 ~ 46
Apatzidou DA / 2005 / Impact of smoking on the clinical, microbiological and immunological parameters of adult patients with periodontitis / J Clin Periodontol 32 : 973 ~ 98
Berglundh T / 2005 / Aspects of adaptive host response in periodontitis / J Clin Periodontol 32 (Suppl 6) : 87 ~ 107
Schindler CW / 2002 / Series introduction. JAK-STAT signaling in human disease / J Clin Invest 109:1133~1137
Alpar B / 1998 / Nicotine-induced alterations in human primary periodontal ligament and gingiva fibroblast cultures / Clin Oral Investig 2 : 40 ~ 46
Apatzidou DA / 2005 / Impact of smoking on the clinical, microbiological and immunological parameters of adult patients with periodontitis / J Clin Periodontol 32 : 973 ~ 98
Berglundh T / 2005 / Aspects of adaptive host response in periodontitis / J Clin Periodontol 32 (Suppl 6) : 87 ~ 107
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