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논문 기본 정보

자료유형
학술저널
저자정보
Euna Park (Pukyong National University) Hae Ok Kim (Kyungnam University) Gyo-Nam Kim (Kyungnam University) Ji-Hye Song (Kyungnam University)
저널정보
한국식품영양과학회 Preventive Nutrition and Food Science Preventive Nutrition and Food Science Vol.20 No.1
발행연도
2015.3
수록면
29 - 37 (9page)

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초록· 키워드

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Most of the wheat germ in cereal grains is removed during the milling process. Various physiological effects have been reported for bioactive substances in wheat germ such as phenolic acids and flavonoids. In this study, the anti-oxidant and anti-adipogenic effects of ethanol extracts from wheat germ (WGE) and wheat germ fermented with Aspergillus oryzae (F-WGE) were investigated in HepG2 and 3T3-L1 cells. The anti-oxidant activity of F-WGE was demonstrated by a dose-dependent increase in the enhanced scavenging capacity of hydroxyl radicals and Cu<SUP>2+</SUP>-chelating activity compared to WGE. WGE and F-WGE treatment at doses between 10 and 400 ?g/mL did not affect the viability of HepG2 and 3T3-L1 cells. Intracellular ROS levels from Cu<SUP>2+</SUP>-induced oxidative stress were significantly decreased by F-WGE treatment in HepG2 cells compared to WGE. Lipid accumulation was increased in 3T3-L1 adipocytes by 100?μM Fe<SUP>2+</SUP> treatment, but the accumulation was strongly inhibited by 100 μg/mL of WGE and F-WGE treatment. These results suggest that changes in bioactive substances during the fermentation of wheat germ can potentiate scavenging activities against transition metal-induced oxidative stress and lipid accumulation in 3T3-L1 adipocytes. Therefore, we propose that F-WGE is a novel food materials and provided scientific evidences for its efficacy in the development of functional foods.

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ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

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UCI(KEPA) : I410-ECN-0101-2016-594-001353400