지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 충북대학교 동물의학연구소 Journal of Biomedical and Translational Research Journal of Biomedical and Translational Research 제21권 제1호
- 발행연도
- 2020.1
- 수록면
- 24 - 29 (6page)
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초록· 키워드
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In previous studies, we found the production of antibodies against cross-reactive bovine serum albumin (BSA) in D-galactose (D-gal) induced aging mouse models. We performed immunoblot analysis with mouse tissue lysates to investigate the changes in the overall autoantibody production in this animal model. And we were able to see the possibility of altering the activity of mouse natural antibodies in this process. In this study, we examined changes in existing natural antibodies in a D-gal-induced aging mouse model. Serum samples were collected from 3-week-old mice (3w), 13-week-old mice (13w), and 13-week-old mice that were treated with D-gal for 6 weeks (13wDG), beginning at the age of 8 weeks. Levels of immunoglobulins (IgM, IgG, and IgA) were quantitatively analyzed in serum samples. Tissue samples were obtained from skin, spleen, and ovary for Western blotting analyses. Natural antibody activity was examined by enzyme-linked immunosorbent assay analyses of anti-double-stranded DNA (dsDNA) antibody. Western blotting analyses using mouse tissue lysates showed that several protein bands detected by serum antibodies from 3w mice became increasingly thicker when detection was performed with serum samples from 13w and 13wDG mice, indicating quantitative increases in levels of natural antibodies. Relative amounts of total IgG, IgM, and IgA immunoglobulins sequentially increased in serum samples from 3w, 13w, and 13wDG mice. A similar tendency was observed regarding the levels of IgM and IgG antibodies against dsDNA. These results indicate increased levels of natural antibodies in the D-gal-induced aging mouse model. Therefore, this animal model could be useful for future natural antibody research.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Ansel KM / 2002 / CXCL13 is required for B1 cell homing, natural antibody production, and body cavity immunity / Immunity 16:67~76
Coutinho A / 1995 / Natural autoantibodies / Curr Opin Immunol 7:812~818
Galili U / 2013 / Anti-Gal : an abundant human natural antibody of multiple pathogeneses and clinical benefits / Immunology 140:1~11
Spalter SH / 1999 / Normal human serum contains natural antibodies reactive with autologous ABO blood group antigens / Blood 93:4418~4424
Baumgarth N / 2005 / Inherent specificities in natural antibodies : a key to immune defense against pathogen invasion / Springer Semin Immunopathol 26:347~362
Coutinho A / 1995 / Natural autoantibodies / Curr Opin Immunol 7:812~818
Galili U / 2013 / Anti-Gal : an abundant human natural antibody of multiple pathogeneses and clinical benefits / Immunology 140:1~11
Spalter SH / 1999 / Normal human serum contains natural antibodies reactive with autologous ABO blood group antigens / Blood 93:4418~4424
Baumgarth N / 2005 / Inherent specificities in natural antibodies : a key to immune defense against pathogen invasion / Springer Semin Immunopathol 26:347~362
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