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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.10
- 수록면
- 734 - 741 (8page)
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초록· 키워드
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Granulocyte-macrophage colony stimulating factor (GM-CSF) has a role in inducing emergency hematopoiesis upon exposure to inflammatory stimuli. Although GM-CSF generated murine bone marrow derived cells have been widely used as macrophages or dendritic cells in research, the exact characteristics of each cell population have not yet been defined. Here we discriminated GM-CSF grown bone marrow derived macrophages (GM-BMMs) from dendritic cells (GM-BMDCs) in several criteria. After C57BL/6J mice bone marrow cell culture for 7 days with GM-CSF supplementation, two main populations were observed in the attached cells based on MHCII and F4/80 marker expressions. GM-BMMs had MHCIIlowF4/80high as well as CD11c+CD11bhighCD80-CD64+MerTK+ phenotypes. In contrast, GM-BMDCs had MHCIIhighF4/80low and CD11chighCD8α-CD11b+CD80+CD64-MerTKlow phenotypes. Interestingly, the GM-BMM population increased but GM-BMDCs decreased in a GM-CSF dose-dependent manner. Functionally, GM-BMMs showed extremely high phagocytic abilities and produced higher IL-10 upon LPS stimulation. GM-BMDCs, however, could not phagocytose as well, but were efficient at producing TNF?, IL-1?, IL-12p70 and IL-6 as well as inducing T cell proliferation. Finally, whole transcriptome analysis revealed that GM-BMMs and GM-BMDCs are overlap with in vivo resident macrophages and dendritic cells, respectively. Taken together, our study shows the heterogeneicity of GM-CSF derived cell populations, and specifically characterizes GM-CSF derived macrophages compared to dendritic cells.
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참고문헌 (27)
참고문헌 신청
Banchereau, J. / 1998 / Dendritic cells and the control of immunity / Nature 392:245~252
Becker, L. / 2012 / Unique proteomic signatures distinguish macrophages and dendritic cells / PLoS One 7:e33297
Bhattacharya, P. / 2011 / GM-CSF-induced, bone-marrow-derived dendritic cells can expand natural Tregs and induce adaptive Tregs by different mechanisms / J. Leukocyte Biol 89:235~249
Cebon, J. / 1994 / Endogenous haemopoietic growth factors in neutropenia and infection / Br. J. Haematol 86:265~274
Cheers, C. / 1988 / Production of colony-stimulating factors(CSFs)during infection : separate determinations of macrophage-, granulocyte-, granulocyte-macrophage-, and multi-CSFs / Infect. Immun 56:247~251
Becker, L. / 2012 / Unique proteomic signatures distinguish macrophages and dendritic cells / PLoS One 7:e33297
Bhattacharya, P. / 2011 / GM-CSF-induced, bone-marrow-derived dendritic cells can expand natural Tregs and induce adaptive Tregs by different mechanisms / J. Leukocyte Biol 89:235~249
Cebon, J. / 1994 / Endogenous haemopoietic growth factors in neutropenia and infection / Br. J. Haematol 86:265~274
Cheers, C. / 1988 / Production of colony-stimulating factors(CSFs)during infection : separate determinations of macrophage-, granulocyte-, granulocyte-macrophage-, and multi-CSFs / Infect. Immun 56:247~251
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