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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 781 - 787 (7page)
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Spermatogenesis is a process which includes the following phases: spermatogonial stem cell proliferation and differentiation, spermatogonia, spermatocyte, spermatid and mature sperm. Spermatogenic failure is the important factor resulting in male infertility. Recent studies showed that long noncoding RNA (lncRNA) have been found to be involved in the regulation of male reproduction. However, lncRNA associated with spermatogenesis and their mechanisms of action are unclear. The aim of this study is to explore the role and molecular mechanism of lncRNA in spermatogenesis. LncRNA microarray of germ cells and bioinformatic analysis showed lncRNA Gm2044 may play potential roles in spermatogenesis. The expression level of RNA and protein were analyzed by RT-qPCR and western blotting, respectively. The interaction of lncRNA with mRNA was detected by RNA pull down and cellular proliferation was measured using CCK-8 reagent. Testis-enriched lncRNA Gm2044 is abundant in mouse spermatocytes. Gm2044 can suppress the translation of adjacent spermatogenesisrelated gene Utf1 by interacting with Utf1 mRNA. Furthermore, the proliferation of mouse spermatogonia GC-1 cell line and spermatocyte GC-2 cell line was inhibited by Gm2044. CONCLUSION: LncRNA Gm2044 was identified to inhibit Utf1 mRNA translation and play important roles in spermatogenesis.
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등록된 정보가 없습니다.
참고문헌 (25)
참고문헌 신청
Agbor VA / 2013 / A Wt1-Dmrt1 transgene restores DMRT1 to sertoli cells of Dmrt1(-/-) testes: a novel model of DMRT1-deficient germ cells / Biol Reprod 88:51
Akhade VS / 2016 / Mechanism of Wnt signaling induced down regulation of mrhl long non-coding RNA in mouse spermatogonial cells / Nucleic Acids Res 44:387~401
Anguera MC / 2011 / Tsx produces a long noncoding RNA and has general functions in the germline, stem cells, and brain / PLoS Genet 7:e1002248
Arun G / 2012 / mrhl RNA, a long noncoding RNA, negatively regulates Wnt signaling through its protein partner Ddx5/p68 in mouse spermatogonial cells / Mol Cell Biol 32:3140~3152
Ayoub N / 1997 / Xist RNA is associated with the transcriptionally inactive XY body in mammalian male meiosis / Chromosoma 106:1~10
Akhade VS / 2016 / Mechanism of Wnt signaling induced down regulation of mrhl long non-coding RNA in mouse spermatogonial cells / Nucleic Acids Res 44:387~401
Anguera MC / 2011 / Tsx produces a long noncoding RNA and has general functions in the germline, stem cells, and brain / PLoS Genet 7:e1002248
Arun G / 2012 / mrhl RNA, a long noncoding RNA, negatively regulates Wnt signaling through its protein partner Ddx5/p68 in mouse spermatogonial cells / Mol Cell Biol 32:3140~3152
Ayoub N / 1997 / Xist RNA is associated with the transcriptionally inactive XY body in mammalian male meiosis / Chromosoma 106:1~10
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