지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.8
- 수록면
- 611 - 618 (8page)
- DOI
- molcells.2016.0103
이용수
초록· 키워드
오류제보하기
Fibroblast-like synoviocytes (FLS) with aberrant expres-sion of microRNA (miRNA) are critical pathogenic regula-tors in rheumatoid arthritis (RA). Previous studies have found that overexpression or silencing of miRNA can contribute to the development of miRNA-based therapeutics in arthritis models. In this study, we explored the effects of miR-27a on cell migration and invasion in cultured FLS from RA patients. We found that miR-27a was markedly downregulated in the serum, synovial tissue, and FLS of RA patients. Meanwhile, the expression of follistatin-like protein 1 (FSTL1) was upregulated, which suggests that FSTL1 plays a key role in RA development. The results of a Transwell assay showed that miR-27a inhibited FLS migration and invasion. However, miR-27a inhibition promoted the migration and invasion of FLS. In addition, the down-regulated expression of matrix metalloproteinases (MMP2, MMP9, and MMP13) and Rho family proteins (Rac1, Cdc42, and RhoA) was detected after treatment with miR-27a in RA-FLS by quantitative reverse transcription-PCR and western blot analysis. Then, a luciferase reporter assay validated that miR-27a targeted the 3-untranslated region (3?-UTR) of FSTL1. Moreover, miR-27a caused a significant decrease of FSTL1. In addition, the expression of TLR4 and NFκB was inhibited by miR-27a but increased by FSTL1 overexpression. In conclusion, we found that miR-27a inhibited cell migration and invasion of RA-FLS by targeting FSTL1 and restraining the TLR4/NFκB pathway.
목차
등록된 정보가 없습니다.
참고문헌 (40)
참고문헌 신청
Abeles, A. M. / 2006 / The role of the synovial fibroblast in rheumatoid arthritis : cartilage destruction and the regulation of matrix metalloproteinases / Bull. NYU Hosp. Jt. Dis 64:20~24
Aletaha, D. / 2010 / 2010 Rheumatoid arthritis classification criteria : an American College of Rheumatology/European League Against Rheumatism collaborative initiative / Arthritis Rheum 62:2569~2581
Androulidaki, A. / 2009 / The kinase Akt1 controls macrophage response to lipopolysaccharide by regulating microRNAs / Immunity 31:220~231
Bottini, N. / 2013 / Duality of fibroblast-like synoviocytes in RA : passive responders and imprinted aggressors / Nat. Rev. Rheumatol 9:24~33
Chaly, Y. / 2012 / FSTL1 promotes arthritis in mice by enhancing inflammatory cytokine/chemokine expression / Arthritis Rheum 64:1082~1088
Aletaha, D. / 2010 / 2010 Rheumatoid arthritis classification criteria : an American College of Rheumatology/European League Against Rheumatism collaborative initiative / Arthritis Rheum 62:2569~2581
Androulidaki, A. / 2009 / The kinase Akt1 controls macrophage response to lipopolysaccharide by regulating microRNAs / Immunity 31:220~231
Bottini, N. / 2013 / Duality of fibroblast-like synoviocytes in RA : passive responders and imprinted aggressors / Nat. Rev. Rheumatol 9:24~33
Chaly, Y. / 2012 / FSTL1 promotes arthritis in mice by enhancing inflammatory cytokine/chemokine expression / Arthritis Rheum 64:1082~1088
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