지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.7
- 수록면
- 676 - 683 (8page)
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Cilia are highly specialized antennae-like organelles that extend from the cell surface and act as cell signaling hubs. Intraflagellar transport (IFT) is a specialized form of intracellular protein trafficking that is required for the assembly and maintenance of cilia. Because cilia are so important, mutations in several IFT components lead to human disease. Thus, clarifying the molecular functions of the IFT proteins is a high priority in cilia biology. Live imaging in various species and cellular preparations has proven to be an important technique in both the discovery of IFT and the mechanisms by which it functions. Live imaging of Drosophila cilia, however, has not yet been reported. Here, we have visualized the movement of IFT in Drosophila cilia using time-lapse live imaging for the first time. We found that NOMPB-GFP (IFT88) moves according to distinct parameters depending on the ciliary segment. NOMPB-GFP moves at a similar speed in proximal and distal cilia toward the tip (~0.45 μm/s). As it returns to the ciliary base, however, NOMPB-GFP moves at ~0.12 μm/s in distal cilia, accelerating to ~0.70 μm/s in proximal cilia. Furthermore, while live imaging NOMPB-GFP, we observed one of the IFT proteins required for retrograde movement, Oseg4 (WDR35), is also required for anterograde movement in distal cilia. We anticipate our time-lapse live imaging analysis technique in Drosophila cilia will be a good starting point for a more sophisticated analysis of IFT and its molecular mechanisms.
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참고문헌 (40)
참고문헌 신청
Avidor-Reiss, T. / 2004 / Decoding cilia function: defining specialized genes required for compartmentalized cilia biogenesis / Cell 117:527~539
Berbari, N.F. / 2009 / The primary cilium as a complex signaling center / Curr. Biol. 19:526~535
Buisson, J. / 2013 / Intraflagellar transport proteins cycle between the flagellum and its base / J. Cell Sci. 126:327~338
Cole, D.G. / 1998 / Chlamydomonas kinesin-II-dependent intraflagellar transport (IFT): IFT particles contain proteins required for ciliary assembly in Caenorhabditis elegans sensory neurons / J. Cell Biol. 141:993~1008
Eberl, D.F. / 2000 / Genetically similar transduction mechanisms for touch and hearing in Drosophila / J. Neurosci. 20:5981~5988
Berbari, N.F. / 2009 / The primary cilium as a complex signaling center / Curr. Biol. 19:526~535
Buisson, J. / 2013 / Intraflagellar transport proteins cycle between the flagellum and its base / J. Cell Sci. 126:327~338
Cole, D.G. / 1998 / Chlamydomonas kinesin-II-dependent intraflagellar transport (IFT): IFT particles contain proteins required for ciliary assembly in Caenorhabditis elegans sensory neurons / J. Cell Biol. 141:993~1008
Eberl, D.F. / 2000 / Genetically similar transduction mechanisms for touch and hearing in Drosophila / J. Neurosci. 20:5981~5988
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