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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 311 - 319 (9page)
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초록· 키워드
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It is very useful to evaluate the content and 3D distribution of extracellular matrix non-destructively in tissue engineering. This study evaluated the feasibility of using micro-computed tomography (lCT) with Hexabrix to measure quantitatively sulfated glycosaminoglycans (GAGs) of engineered cartilage. Rabbit chondrocytes at passage 2 were used to produce artificial cartilages in polyglycolic acid scaffolds in vitro. Engineered cartilages were incubated with Hexabrix 320 for 20 min and analyzed via lCT scanning. The number of voxels in the 2D and 3D scanning images were counted to estimate the amount of sulfated GAGs. The optimal threshold value for quantification was determined by regression analysis. The 2D lCT images of an engineered cartilage showed positive correlation with the histological image of Safranin-O staining. Quantitative data obtained with the 3D lCT images of 14 engineered cartilages showed strong correlation with sulfated GAGs contents obtained by biochemical analysis (R2 = 0.883, p\0.001). Repeated exposure of engineered cartilages to Hexabrix 320 and lCT scanning did not significantly affect cell viability, total DNA content, or the total content of sulfated GAGs. We conclude that lCT imaging using Hexabrix 320 provides high spatial resolution and sensitivity to assess the content and 3D distribution of sulfated GAGs in engineered cartilages. It is expected to be a valuable tool to evaluate the quality of engineered cartilage for commercial development in the future.
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참고문헌 (20)
참고문헌 신청
Daiki Murata / 2018 / Osteochondral Regeneration with a Scaffold-Free Three-Dimensional Construct of Adipose Tissue-Derived Mesenchymal Stromal Cells in Pigs / 조직공학과 재생의학 15(1):101~113
정치성 / 2018 / Development of Printable Natural Cartilage Matrix Bioink for 3D Printing of Irregular Tissue Shape / 조직공학과 재생의학 15(2):155~162
Jin CZ / 2007 / In vivo cartilage tissue engineering using a cell-derived extracellular matrix scaffold / Artif Organs 31:183~192
Jin RL / 2009 / Scaffold-free cartilage fabrication system using passaged porcine chondrocytes and basic fibroblast growth factor / Tissue Eng Part A 15:1887~1895
Buckwalter JA / 2005 / Articular cartilage and osteoarthritis / Instr Course Lect 54:465~480
정치성 / 2018 / Development of Printable Natural Cartilage Matrix Bioink for 3D Printing of Irregular Tissue Shape / 조직공학과 재생의학 15(2):155~162
Jin CZ / 2007 / In vivo cartilage tissue engineering using a cell-derived extracellular matrix scaffold / Artif Organs 31:183~192
Jin RL / 2009 / Scaffold-free cartilage fabrication system using passaged porcine chondrocytes and basic fibroblast growth factor / Tissue Eng Part A 15:1887~1895
Buckwalter JA / 2005 / Articular cartilage and osteoarthritis / Instr Course Lect 54:465~480
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