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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 1,181 - 1,197 (17page)
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Tropical plant rubber tree (Hevea brasiliensis) is the sole source of commercial natural rubber and low-temperature stress is the most important limiting factor for its cultivation. To characterize the gene expression profiles of H. brasiliensis under the cold stress and discover the key cold stress-induced genes. Three cDNA libraries, CT (control), LT2 (cold treatment at 4 °C for 2 h) and LT24 (cold treatment at 4 °C for 24 h) were constructed for RNA sequencing (RNA-Seq) and gene expression profiling. Quantitative real time PCR (qRT-PCR) was conducted to validate the RNA-Seq and gene differentially expression results. A total of 1457 and 2328 differentially expressed genes (DEGs) in LT2 and LT24 compared with CT were respectively detected. Most significantly enriched KEGG pathways included flavonoid biosynthesis, phenylpropanoid biosynthesis, plant hormone signal transduction, cutin, suberine and wax biosynthesis, Pentose and glucuronate interconversions, phenylalanine metabolism and starch and sucrose metabolism. A total of 239 transcription factors (TFs) were differentially expressed following 2 h or/and 24 h of cold treatment. Cold-response transcription factor families included ARR-B, B3, BES1, bHLH, C2H, CO-like, Dof, ERF, FAR1, G2-like, GRAS, GRF, HD-ZIP, HSF, LBD, MIKC-MADS, M-type MADS, MYB, MYB-related, NAC, RAV, SRS, TALE, TCP, Trihelix, WOX, WRKY, YABBY and ZF-HD. The genome-wide transcriptional response of rubber tree to the cold treatments were determined and a large number of DEGs were characterized including 239 transcription factors, providing important clues for further elucidation of the mechanisms of cold stress responses in rubber tree.
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참고문헌 (5)
참고문헌 신청
Jian Ding / 2017 / Comparative transcriptome analysis of lipid biosynthesis in seeds and non-seed tissues of sea buckthorn / Genes & Genomics 39(9):1021~1033
Wenping Hua / 2017 / Transcriptome analysis of Dioscorea zingiberensis identifies genes involved in diosgenin biosynthesis / Genes & Genomics 39(5):509~520
Jiajia Li / 2017 / Transcriptome comparative analysis between the cytoplasmic male sterile line and fertile line in soybean (Glycine max (L.) Merr.) / Genes & Genomics 39(10):1117~1127
Peng Wang / 2017 / Transcriptome analysis reveals sunflower cytochrome P450 CYP93A1 responses to high salinity treatment at the seedling stage / Genes & Genomics 39(6):581~591
Xicheng Wang / 2017 / Comparative transcriptome analysis of berry-sizing effects of gibberellin ( GA3) on seedless Vitis vinifera L. / Genes & Genomics 39(5):493~507
Wenping Hua / 2017 / Transcriptome analysis of Dioscorea zingiberensis identifies genes involved in diosgenin biosynthesis / Genes & Genomics 39(5):509~520
Jiajia Li / 2017 / Transcriptome comparative analysis between the cytoplasmic male sterile line and fertile line in soybean (Glycine max (L.) Merr.) / Genes & Genomics 39(10):1117~1127
Peng Wang / 2017 / Transcriptome analysis reveals sunflower cytochrome P450 CYP93A1 responses to high salinity treatment at the seedling stage / Genes & Genomics 39(6):581~591
Xicheng Wang / 2017 / Comparative transcriptome analysis of berry-sizing effects of gibberellin ( GA3) on seedless Vitis vinifera L. / Genes & Genomics 39(5):493~507
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