지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 1,117 - 1,127 (11page)
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To further elucidate the molecular mechanism and fertility restoration of cytoplasmic male sterility (CMS) in soybean, a comparative transcriptome analysis was conducted between the CMS line NJCMS1A, restorer line NJCMS1C and their hybrid F1 progeny (NJCMS1A × NJCMS1C) using RNA-Seq strategy. After pairwise comparative analysis of these soybean lines, 294, 222, and 288 differentially expressed genes (DEGs) were identified, respectively. Further bioinformatic analysis indicated that these DEGs were involved in diverse molecular functions and metabolic pathways. qRT-PCR analysis validated that the gene expression pattern in RNA-Seq was reliable. These results significantly showed that the male sterility and fertility restoration in NJCMS1A might be related to a series of the abnormal of growth development and metabolic processes, such as pollen development, DNA methylation process, pollen viability, cell wall development, programmed cell death, as well as carbohydrate and energy metabolism. This study could facilitate our understanding of the molecular mechanisms and fertility restoration behind CMS in soybean.
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참고문헌 (42)
참고문헌 신청
An H / 2014 / Comparative transcript profiling of the fertile and sterile flower buds of pol CMS in B. napus / BMC Genomics 15:258
Ansorge WJ / 2009 / Next-generation DNA sequencing techniques / New Biotechnol 25:195~203
Benjamini BY / 1995 / Controlling the false discovery rate: a practical and powerful approach to multiple testing / J R Stat Soc 57:289~300
Benjamini BY / 2001 / The control of the false discovery rate in multiple testing under dependency / Ann Stat 29:1165~1188
Conesa A / 2005 / Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research / Bioinformatics 21:3674~3676
Ansorge WJ / 2009 / Next-generation DNA sequencing techniques / New Biotechnol 25:195~203
Benjamini BY / 1995 / Controlling the false discovery rate: a practical and powerful approach to multiple testing / J R Stat Soc 57:289~300
Benjamini BY / 2001 / The control of the false discovery rate in multiple testing under dependency / Ann Stat 29:1165~1188
Conesa A / 2005 / Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research / Bioinformatics 21:3674~3676
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