Transcriptome analysis of Dioscorea zingiberensis identifies genes involved in diosgenin biosynthesis

Wenping Hua; Weiwei Kong; XiaoYan Cao; Chen Chen; Qian Liu; Xiangmin Li; Zhezhi Wang

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자료유형: 학술저널

저자정보: Wenping Hua (Shaanxi Normal University) Weiwei Kong (Shaanxi Normal University) XiaoYan Cao (Shaanxi Normal University) Chen Chen (Shaanxi Normal University) Qian Liu (Shaanxi Normal University) Xiangmin Li (Shaanxi Normal University) Zhezhi Wang (Shaanxi Normal University)

저널정보: 한국유전학회 Genes & Genomics Genes & Genomics Vol.39 No.5

발행연도: 2017.1

수록면: 509 - 520 (12page)

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Dioscorea zingiberensis is the main plant source of diosgenin, a precursor for the production of steroid hormones used in the pharmaceutical industry. The extraction process of diosgenin from D. zingiberensis can generate high-acid and high-strength wastewater on a large scale and can threaten the environment. Bioengineering microorganisms to produce diosgenin is an effective way to avoid pollution. However, little is known about the genes that are involved in the biosynthesis of diosgenin. We obtained 85,010 unigenes (average length of 1142 bases) from the D. zingiberensis transcriptome through RNAseq. A large number of unigenes (59,368; 69.83%) were annotated, and 2488 unigenes were assigned to 27 secondary- metabolite pathways. In our database, 66 unigenes encoding up to 40 key enzymes were found to be present in diosgenin biosynthesis pathways. In addition, we found 203 unigenes encoding CYP450 proteins and 47 unigenes encoding UGT proteins that may be involved in modifications of a downstream pathway. The expression patterns of key diosgenin biosynthesis genes were studied to identify the most important members of the enzyme family. These results add to the available genetic data of D. zingiberensis and lay the foundation for the further production of diosgenin using genetic engineering.

참고문헌 (56)

참고문헌 신청

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