지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 493 - 507 (15page)
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초록· 키워드
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Gibberellin (GA) is widely used to enlarge berries of seedless table grape and raisin varieties. The molecular mechanism underlying the berry-sizing effect of gibberelins is however poorly understood. We used a high-throughput deep sequencing technology to investigate the transcriptome and comparative expression profiles of berries from Vitis vinifera L. ‘Summer Black’ treated with GA3 with the aim of increasing the understanding of molecular mechanisms underlying the species’ expansion growth responses to exogenous GA3 hormone application. A total of 591 differentially expressed genes (DEGs) were detected including genes involved in fruit expansion and growth. There were four expansion genes, three cellulose synthase A catalytic subunit genes, four cellulose synthaselike protein genes and three xyloglucan endotransglucosylase genes. Differential expression of these genes could potentially explain the difference in the growth and sizes of fruits from control (CK) and GA3 treated (+GA) vines. In addition, the expression patterns of 14 DEGs were validated by qRT-PCR, and the outcomes agreed highly with the RNA-Seq results. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed to better understand the functions of these DEGs. We also identified a large number of single nucleotide polymorphism and insertion/deletion markers, which will be a rich resource for future marker development and breeding research in grape. The transcriptome analysis provides valuable information for furthering our understanding of the molecular mechanisms that regulate the fruit expansion growth, and adds to the growing foundation for future genetic and functional genomic studies in grape fruit.
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참고문헌 (76)
참고문헌 신청
Alagna F / 2009 / Comparative 454 pyrosequencing of transcripts from two olive genotypes during fruit development / BMC Genom 10:399
Albert M / 2004 / The cell wall-modifying xyloglucan endotransglycosylase/hydrolase LeXTH1 is expressed during the defence reaction of tomato against the plant parasite Cuscuta reflexa / Plant Biol 6:402~407
Anders S / 2010 / Differential expression analysis for sequence count data / Genome Biol 11:R106
Benjamini Y / 2001 / The control of the false discovery rate in multiple testing under dependency / Ann Stat 29:1165~1188
Blencowe BJ / 2006 / Alternative splicing: new insights from global analyses / Cell 126:37~47
Albert M / 2004 / The cell wall-modifying xyloglucan endotransglycosylase/hydrolase LeXTH1 is expressed during the defence reaction of tomato against the plant parasite Cuscuta reflexa / Plant Biol 6:402~407
Anders S / 2010 / Differential expression analysis for sequence count data / Genome Biol 11:R106
Benjamini Y / 2001 / The control of the false discovery rate in multiple testing under dependency / Ann Stat 29:1165~1188
Blencowe BJ / 2006 / Alternative splicing: new insights from global analyses / Cell 126:37~47
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