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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 1,157 - 1,167 (11page)
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Sulfolobus species can grow on a variety of organic compounds as carbon and energy sources. These species degrade glucose to pyruvate by the modified branched Entner–Doudoroff pathway. We attempted to determine the differentially expressed genes (DEGs) under sugar-limited and sugar-rich conditions. RNA sequencing (RNA-seq) was used to quantify the expression of the genes and identify those DEGs between the S. acidocaldarius cells grown under sugar-rich (YT with glucose) and sugar-limited (YT only) conditions. The functions and pathways of the DEGs were examined using gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. Quantitative real-time PCR (qRT-PCR) was performed to validate the DEGs. Transcriptome analysis of the DSM 639 strain grown on sugar-limited and sugar-rich media revealed that 853 genes were differentially expressed, among which 481 were upregulated and 372 were downregulated under the glucose-supplemented condition. In particular, 70 genes showed significant changes in expression levels of ≥ twofold. GO and KEGG enrichment analyses revealed that the genes encoding components of central carbon metabolism, the respiratory chain, and protein and amino acid biosynthetic machinery were upregulated under the glucose condition. RNA-seq and qRT-PCR analyses indicated that the sulfur assimilation genes (Saci_2197–2204) including phosphoadenosine phosphosulfate reductase and sulfite reductase were significantly upregulated in the presence of glucose. The present study revealed metabolic networks in S. acidocaldarius that are induced in a glucose-dependent manner, improving our understanding of biomass production under sugar-rich conditions.
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참고문헌 (38)
참고문헌 신청
Berg IA / 2010 / Study of the distribution of autotrophic CO2 fixation cycles in Crenarchaeota / Microbiology 156:256~269
Borirak O / 2015 / Time-series analysis of the transcriptome and proteome of Escherichia coli upon glucose repression / Biochim Biophys Acta 1854:1269~1279
Brasen C / 2014 / Carbohydrate metabolism in Archaea : current insights into unusual enzymes and pathways and their regulation / Microbiol Mol Biol Rev 78:89~175
Brock TD / 1972 / Sulfolobus : a new genus of sulfur-oxidizing bacteria living at low pH and high temperature / Arch Mikrobiol 84:54~68
Chen L / 2005 / The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota / J Bacteriol 187:4992~4999
Borirak O / 2015 / Time-series analysis of the transcriptome and proteome of Escherichia coli upon glucose repression / Biochim Biophys Acta 1854:1269~1279
Brasen C / 2014 / Carbohydrate metabolism in Archaea : current insights into unusual enzymes and pathways and their regulation / Microbiol Mol Biol Rev 78:89~175
Brock TD / 1972 / Sulfolobus : a new genus of sulfur-oxidizing bacteria living at low pH and high temperature / Arch Mikrobiol 84:54~68
Chen L / 2005 / The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota / J Bacteriol 187:4992~4999
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