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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 349 - 359 (11page)
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Cryptochromes, a class of blue light photoreceptors, play vital roles in regulating growth and development in higher plants. Despite their control over various important traits, there have been few studies focusing on cryptochromes in forest trees to date. In this study, the Euphrates poplar (Populus euphratica) gene PeCRY1 (cryptochrome 1 of Populus euphratica) was isolated and heterologously expressed in Populus tomentosa. Three biological replicates of each of the PeCRY1 transgenic P. tomentosa (CRY1) and wild-type (WT) plants were processed for transcriptome profiling. We found 34792 commonly expressed transcripts among the 93868 detected unigenes. Using R package DESeq, we identified 357 differentially expressed genes (DEGs), including 132 upregulated and 225 downregulated genes. Gene ontology (GO) enrichment and KEGG pathway enrichment were used to better understand the functions of detected DEGs. Biosynthetic processes, such as starch and sucrose metabolism, which are closely related to growth and development, were highly enriched. Organic cyclic compound biosynthesis was downregulated, whereas carbohydrate metabolism was upregulated. Through KEGG pathway enrichment, we observed that the pentose phosphate pathway, photosynthesis, and circadian rhythm were significantly enriched. Another method of expression analysis based on quantitative reverse transcription polymerase chain reaction (qRT-PCR) validated our RNA sequencing (RNA-seq) results.
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참고문헌 (59)
참고문헌 신청
Ahmad M / 1993 / HY4 gene of A. thaliana encodes a protein with characteristics of a blue-light photoreceptor / Nature 366:162~166
Ahmad M / 1995 / Mutations throughout an Arabidopsis blue-light photoreceptor impair blue-light-responsive anthocyanin accumulation and inhibition of hypocotyl elongation / Plant J 8:653~658
Ahmad M / 2007 / Magnetic intensity affects cryptochrome-dependent responses in Arabidopsis thaliana / Planta 225:615~624
Al-Sady B / 2006 / Photoactivated phytochrome induces rapid PIF3 phosphorylation prior to proteasome-mediated degradation / Mol Cell 23:439~446
Altschul SF / 1997 / Gapped BLAST and PSI-BLAST: a new generation of protein database search programs / Nucl Acids Res 25:3389~3402
Ahmad M / 1995 / Mutations throughout an Arabidopsis blue-light photoreceptor impair blue-light-responsive anthocyanin accumulation and inhibition of hypocotyl elongation / Plant J 8:653~658
Ahmad M / 2007 / Magnetic intensity affects cryptochrome-dependent responses in Arabidopsis thaliana / Planta 225:615~624
Al-Sady B / 2006 / Photoactivated phytochrome induces rapid PIF3 phosphorylation prior to proteasome-mediated degradation / Mol Cell 23:439~446
Altschul SF / 1997 / Gapped BLAST and PSI-BLAST: a new generation of protein database search programs / Nucl Acids Res 25:3389~3402
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