LncRNA expression profile analysis of Mg2+-induced osteogenesis by RNA-seq and bioinformatics

Tang Wen; Liu Qing; Tan Wei; Sun Tianshi; Deng Youwen

doi:10.1007/s13258-021-01140-w

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자료유형: 학술저널

저자정보: Tang Wen (Third Xiangya Hospital Central South University) Liu Qing (Third Xiangya Hospital Central South University) Tan Wei (Third Xiangya Hospital Central South University) Sun Tianshi (Third Xiangya Hospital Central South University) Deng Youwen (Third Xiangya Hospital Central South University)

저널정보: 한국유전학회 Genes & Genomics Genes & Genomics Vol.43 No.11

발행연도: 2021.11

수록면: 1,247 - 1,257 (11page)

DOI: 10.1007/s13258-021-01140-w

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Background In recent years, magnesium (Mg) has been extensively studied for manufacturing biodegradable orthopedic devices. Besides other advantages, researches have shown that magnesium-based implants can stimulate osteogenesis thus accelerating orthopedic trauma recovery, but its molecular mechanism is not fully understood. Meanwhile, long non-coding RNA (lncRNA) has been found to play vital role in regulating osteogenic diferentiation. Objective To explore the role of lncRNA in Mg2+ (magnesium ions)-induced osteogenesis. Methods The efect of Mg2+ on mBMSCs proliferation was detected by the CCK-8 assay. The optimum concentration of Mg2+ (7.5 mM) in promoting mBMSCs osteogenesis was determined by ALP staining and Alizarin red staining, western blot and RT-qPCR were performed to detect osteogenic markers expressions. The lncRNAs and mRNAs expression profles of mBMSCs were assessed by RNA-Seq and processed by bioinformatics analysis. The selected lncRNAs expression level was validated by RT-qPCR. Results The efect of Mg2+ in promoting osteogenesis was confrmed and the optimum concentration was determined as 7.5 mM. The lncRNAs and mRNAs diferentially expressed between 7.5 mM Mg2+-treated group and control group was detected and functional analysis revealed that their function were associated with osteogenesis. The ceRNA networks were constructed for H19 and Dubr that aberrantly expressed in two groups. The ceRNA networks of selected lncRNAs (H19 and Dubr) were constructed. Conclusions This study identifed H19 and Dubr as osteogenic associated lncRNAs involved in Mg2+-induced osteogenesis, and they might play their roles through lncRNA-miRNA?mRNA axis.

#Magnesium #Osteogenesis #H19 long non-coding RNA #Mesenchymal stem cells

참고문헌 (55)

참고문헌 신청

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