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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 1,047 - 1,057 (11page)
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microRNAs (miRNAs) and PIWI-interacting RNAs (piRNAs) execute important regulatory roles in testis development and spermatogenesis, while previous studies mainly focus on the expression profiles in immature and mature porcine testes, which may cause a bottleneck for further understanding their complex physiological processes in porcine testes development and spermatogenesis. Thus, we presented the expression and characterization of miRNAs and piRNAs in DS (60-day-old), DN (90-dayold), DT (120-day-old) and DF (150-day-old) pig testes. In total, 12,834,628, 13,359,726, 12,851,249 and 12,938,601 clean reads were generated from these libraries, respectively. 293 mature and 36 novel miRNAs as well as 4923 piRNA clusters were identified from pig testes, and they showed an age-dependent manner. GO enrichment analysis of miRNA target genes and piRNA generated genes showed that they participated widely in regulating the pig spermatogenesis process. In addition, 12 differentially expressed miRNAs were randomly selected to validate using qRT-PCR. Our results provided novel comprehensive expression profiles of miRNAs and piRNAs in pig testes at different stages of sexual maturity, which will promote our knowledge of them in regulating the pig testes development and spermatogenesis process.
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참고문헌 (42)
참고문헌 신청
Aravin A / 2006 / A novel class of small RNAs bind to MILI protein in mouse testes / Nature 442:203~207
Beyret E / 2012 / piRNA biogenesis during adult spermatogenesis in mice is independent of the ping-pong mechanism / Cell Res 22:1429~1439
Busk PK / 2014 / A tool for design of primers for microRNA-specific quantitative RT-qPCR / BMC Bioinform 15:29
Cora E / 2014 / The MID-PIWI module of Piwi proteins specifies nucleotide- and strand-biases of piRNAs / RNA 20:773~781
Cui L / 2015 / Spermatozoa micro ribonucleic acid-34c level is correlated with intracytoplasmic sperm injection outcomes / Fertil Steril 104(312?7):e1
Beyret E / 2012 / piRNA biogenesis during adult spermatogenesis in mice is independent of the ping-pong mechanism / Cell Res 22:1429~1439
Busk PK / 2014 / A tool for design of primers for microRNA-specific quantitative RT-qPCR / BMC Bioinform 15:29
Cora E / 2014 / The MID-PIWI module of Piwi proteins specifies nucleotide- and strand-biases of piRNAs / RNA 20:773~781
Cui L / 2015 / Spermatozoa micro ribonucleic acid-34c level is correlated with intracytoplasmic sperm injection outcomes / Fertil Steril 104(312?7):e1
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