Characterization and differential expression of microRNA in skeletal muscle of Laiwu and Yorkshire pig breeds

Wei Chen; Guo-Feng Fang; Shou-Dong Wang; Hui Wang; Yong-Qing Zeng

추천

검색

자료유형: 학술저널

저자정보: Wei Chen (Shandong Agricultural University) Guo-Feng Fang (Shandong Agricultural University) Shou-Dong Wang (Shandong Agricultural University) Hui Wang (Shandong Agricultural University) Yong-Qing Zeng (Shandong Agricultural University)

저널정보: 한국유전학회 Genes & Genomics Genes & Genomics Vol.39 No.2

발행연도: 2017.1

수록면: 173 - 182 (10page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

The domestic pig is an important agricultural animal used as a source of meat worldwide. As an important resource, Laiwu pig has some special characteristics compared with Yorkshire pig. It is necessary to identify the differentially expressed microRNAs (miRNAs) in skeletal muscle between two pig breeds. Therefore, we herein performed a comprehensive investigation for miRNA transcriptome of skeletal muscle from the two pig breeds. On average, we obtained approximately 13,493,607 clean reads in Laiwu pig and 12,938,257 clean reads in Yorkshire pig in this study. Totally, 265 known miRNAs were identified, and among these, 229 miRNAs were expressed in all the six pigs. From the 265 known miRNAs, 19 of these miRNAs were found significantly differentially expressed (q\0.05). Of these significantly expressed miRNAs, seven miRNAs were significantly upregulated and 12 miRNAs were down-regulated in Laiwu pig compared with Yorkshire pig. Among the significantly expressed miRNAs, ssc-miR-194-5p has the largest foldchange (6.35-fold). Ten differentially expressed miRNAs selected from high throughput sequencing were confirmed by real-time RT-PCR (RT-qPCR), and the results indicated that the expression patterns were consistent with sequencing results. Our results not only present a comprehensive miRNA transcriptome profile of skeletal muscle between two pig breeds with distinct phenotypes, but also could be useful for investigating the functions of differentially expressed miRNAs associated with the phenotype traits.

참고문헌 (32)

참고문헌 신청

Ambros V / 2004 / The functions of animal microRNAs / Nature 431:350~355

Bartel DP / 2004 / MicroRNAs: genomics, biogenesis, mechanism, and function / Cell 116:281~297

Benjamini Y / 1995 / Controlling the false discovery rate:a practical and powerful approach to multiple testing / J R Stat Soc B 57:289~300

Betel D / 2010 / Comprehensive modeling of microRNA targets predicts functional nonconserved and non-canonical sites / Genome Biol 11:R90

Bushati N / 2007 / microRNA Functions / Annu Rev Cell Dev Biol 23:175~205

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!