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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 543 - 551 (9page)
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Background MicroRNAs (miRNAs) could regulate the expression of target genes and play important roles in modulation of various metabolic processes. Nevertheless, little is known about the backfat microRNAome (miRNAome) of the Neijiang pig. Objectives The primary objective of this study was to analyse miRNAomes of Landrace and Neijiang pig backfat (LPB and NPB resp.). Furthermore, investigating differentially expressed miRNAs participating in lipid metabolism and mining potential biomarker for Neijiang pig breeding. Methods Here we used the Landrace pig with different metabolic characteristics as a control to analyse the Neijiang pig-specific backfat miRNAome. A comprehensive analysis of miRNAomes was performed by deep sequencing. Results Small RNA sequencing identified 326 unique miRNAs, 280 were co-expressed in both libraries. Only 11 and 35 miRNAs were specifically expressed in LPB and NPB respectively. Sixty seven differentially expressed miRNAs were identified by IDEG6. MiR-1-3p were identified that may participate in lipid metabolism. Furthermore, qPCR results revealed that lower expression of miR-1-3p in NPB could increase the expression of LXRα, which is an enzyme important for the synthesis and accumulation of lipid. The double luciferase report experiment suggested that LXRα was the direct target gene of miR-1-3p. In short, miR-1-3p could modulate the synthesis and accumulation of lipid by target LXRα. It may be a potential marker for pig breeding. Conclusion Our investigation has delineated the different miRNAs expression patterns of LPB and NPB, which may help understand the regulatory mechanisms of miRNAs in the lipid metabolism, and provide potential biomarkers for Neijiang pig breeding.
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참고문헌 (43)
참고문헌 신청
Akhlaq Ahmad / 2018 / Tumor-suppressive miRNA-135a inhibits breast cancer cell proliferation by targeting ELK1 and ELK3 oncogenes / Genes & Genomics 40(3):243~251
Bellingham SA / 2012 / Small RNA deep sequencing reveals a distinct miRNA signature released in exosomes from prion-infected neuronal cells / Nucleic Acids Res 21:10937~10949
Bu H / 2000 / RYR1 genotype of the Chinese Neijiang pig / Transpl Proc 32:1058
Carthew RW / 2009 / Origins and mechanisms of miRNAs and siRNAs / Cell 136:642~655
Chan S-P / 2006 / Point of View microRNA-mediated silencing inside P-bodies / RNA Biol 3:97~100
Bellingham SA / 2012 / Small RNA deep sequencing reveals a distinct miRNA signature released in exosomes from prion-infected neuronal cells / Nucleic Acids Res 21:10937~10949
Bu H / 2000 / RYR1 genotype of the Chinese Neijiang pig / Transpl Proc 32:1058
Carthew RW / 2009 / Origins and mechanisms of miRNAs and siRNAs / Cell 136:642~655
Chan S-P / 2006 / Point of View microRNA-mediated silencing inside P-bodies / RNA Biol 3:97~100
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