Identification and comparative analysis of the pearl oyster Pinctada fucata hemocytes microRNAs in response to Vibrio alginolyticus infection

Zhongliang Wang; Bei Wang; Gang Chen; Yishan Lu; Jichang Jian; Zaohe Wu

추천

검색

자료유형: 학술저널

저자정보: Zhongliang Wang (Guangdong Ocean University) Bei Wang (Guangdong Ocean University) Gang Chen (Guangdong Ocean University) Yishan Lu (Guangdong Ocean University) Jichang Jian (Guangdong Ocean University) Zaohe Wu (Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Ani)

저널정보: 한국유전학회 Genes & Genomics Genes & Genomics Vol.39 No.10

발행연도: 2017.1

수록면: 1,069 - 1,081 (13page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

MicroRNAs (miRNAs) are a class of noncoding RNA molecules that function as negative regulators of gene expression and play important roles in a wide spectrum of biological processes, including in immune response. However, the physiological regulation function of Pinctada fucata miRNAs, specially their immunomodulation has not been explored yet. Here, two small RNA libraries from hemocytes of P. fucata with or without Vibrio alginolyticus infection were constructed and sequenced using the high-throughput Illumina deep sequencing technology. In total, 11,939,992 and 11,083,327 raw reads, corresponding to 10,993,546 and 9,988,179 clean reads, were respectively obtained in the control and infected libraries. A total of 276 miRNAs, including 225 known miRNAs and 51 putative novel miRNAs, were identified by bioinformatic analysis. By using pairwise comparison between two libraries, 93 miRNAs were found to be significantly differentially expressed, with 42 and 51 miRNAs exhibiting up-regulation and down-regulation, respectively. Thereinto, some known miRNAs were considered to be immune-related. Real-time PCR were implemented for 6 miRNAs co-expressed in the control and infected samples, and agreement was confirmed between the high-throughput sequencing and real-time PCR data. After miRNA targets were predicted, GO and KEGG pathway enrichment analysis were performed, and the results indicated that ten of the differentially expressed miRNAs were involved in immunerelated pathways, and might participate in the host immune response to V. alginolyticus. These results of identification and comparative analysis of miRNAs might deepen our understanding of host-pathogen interactions and immune defense mechanisms in P. fucata.

참고문헌 (91)

참고문헌 신청

Aboobaker AA / 2005 / Drosophila microRNAs exhibit diverse spatial expression patterns during embryonic development / Proc Natl Acad Sci USA 102:18017~18022

Ambros V / 2004 / The functions of animal microRNAs / Nature 431:350~355

Bao Y / 2014 / Identification and comparative analysis of the Tegillarca granosa haemocytes microRNA transcriptome in response to Cd using a deep sequencing approach / PLoS ONE 9:e93619

Barozai MY / 2012 / Identification and characterization of the microRNAs and their targets in Salmo salar / Gene 499:163~168

Bartel DP / 2004 / MicroRNAs: genomics, biogenesis, mechanism, and function / Cell 116:281~297

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!