지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 81 - 90 (10page)
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초록· 키워드
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BACKGROUND: Cell-based therapies have been studied for articular cartilage regeneration. Articular cartilage defects have little treatments because articular cartilage was limited regenerative capacity. Damaged articular cartilage is difficult to obtain a successful therapeutic effect. In additionally these articular cartilage defects often cause osteoarthritis. Chondrocyte implantation is a widely available therapy used for regeneration of articular cartilage because this tissue has poor repair capacity after injury. Human nasal septum-drived chondrocytes (hNCs) from the septum show greater proliferation ability and chondrogenic capacity than human articular chondrocytes (hACs), even across different donors with different ages. Moreover, the chondrogenic properties of hNCs can be maintained after extensive culture expansion. METHODS: In this study, 2 dimensional (2D) monolayer cultured hNCs (hNCs-2D) and 3 dimensional (3D) spheroids cultured hNCs (hNCs-3D) were examined for chondrogenic capacity in vitro by PCR and immunofluorescence staining for chondrogenic marker, cell survival during cultured and for cartilage regeneration ability in vivo in a rat osteochondral defect model. RESULTS: hNCs-3D showed higher viability and more uniform morphology than 3D spheroids cultured hACs (hACs- 3D) in culture. hNCs-3D also showed greater expression levels of the chondrocyte-specific marker Type II collagen (COL2A1) and sex-determining region Y (SRY)-box 9 (SOX9) than hNCs-2D. hNCs-3D also expressed chondrogenic markers in collagen. Specially, in the osteochondral defect model, implantation of hNCs-3D led to greater chondrogenic repair of focal cartilage defects in rats than implantation of hNCs-2D. CONCLUSION: These data suggest that hNCs-3D are valuable therapeutic agents for repair and regeneration of cartilage defects.
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참고문헌 (45)
참고문헌 신청
Brittberg M / 1994 / Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation / N Engl J Med 331:889~895
Demoor M / 2014 / Cartilage tissue engineering : molecular control of chondrocyte differentiation for proper cartilage matrix reconstruction / Biochim Biophys Acta 1840:2414~2440
Darling EM / 2005 / Rapid phenotypic changes in passaged articular chondrocyte subpopulations / J Orthop Res 23:425~432
Holtzer H / 1960 / The loss of phenotypic traits by differentiated cells in vitro, I. dedifferentiation of cartilage cells / Proc Natl Acad Sci U S A. 46:1533~1542
Kempson GE / 1982 / Relationship between the tensile properties of articular cartilage from the human knee and age / Ann Rheum Dis 41:508~511
Demoor M / 2014 / Cartilage tissue engineering : molecular control of chondrocyte differentiation for proper cartilage matrix reconstruction / Biochim Biophys Acta 1840:2414~2440
Darling EM / 2005 / Rapid phenotypic changes in passaged articular chondrocyte subpopulations / J Orthop Res 23:425~432
Holtzer H / 1960 / The loss of phenotypic traits by differentiated cells in vitro, I. dedifferentiation of cartilage cells / Proc Natl Acad Sci U S A. 46:1533~1542
Kempson GE / 1982 / Relationship between the tensile properties of articular cartilage from the human knee and age / Ann Rheum Dis 41:508~511
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