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논문 기본 정보

자료유형
학술저널
저자정보
Do Hyun Kim (College of Medicine The Catholic University of Korea) Mi Hyun Lim (College of Medicine The Catholic University of Korea) Jung Ho Jeun (College of Medicine The Catholic University of Korea) Sun Hwa Park (College of Medicine The Catholic University of Korea) WeonSun Lee (College of Medicine The Catholic University of Korea) Sang Hi Park (The Catholic University of Korea) Mi Yeon Kwon (College of Medicine The Catholic University of Korea) Se-Hwan Hwang (Bucheon St. Mary's Hospital) Sung Won Kim (The Catholic University of Korea)
저널정보
한국조직공학과 재생의학회 조직공학과 재생의학 조직공학과 재생의학 제16권 제6호
발행연도
2019.1
수록면
605 - 614 (10page)

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BACKGROUND: In this study, we manufactured a complex of human nasal septal cartilage (hNC) with polycaprolactone (PCL) for transplantation into cartilaginous skeletal defects and evaluated their characteristics. METHODS: Nasal septum tissue was obtained from five patients aged C 20 years who were undergoing septoplasty. hNCs were isolated and subcultured for three passages in vitro. To formulate the cell–PCL complex, we used type I collagen as an adhesive between chondrocyte and PCL. Immunofluorescence staining, cell viability and growth in the hNC–PCL complex, and mycoplasma contamination were assessed. RESULTS: hNCs in PCL showed viability C 70% and remained at these levels for 9 h of incubation at 4 C. Immunostaining of the hNC–PCL complex also showed high expression levels of chondrocyte-specific protein, COL2A1, SOX9, and aggrecan during 24 h of clinically applicable conditions. CONCLUSION: The hNC–PCL complex may be a valuable therapeutic agent for implantation into injured cartilage tissue, and can be used clinically to repair cartilaginous skeletal defects. From a clinical perspective, it is important to set the short duration of the implantation process to achieve effective functional implantation.

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