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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 163 - 172 (10page)
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초록· 키워드
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For the cartilage repair, the cell sources currently adopted are primarily chondrocytes or mesenchymal stem cells (MSCs). Due to the fact that chondrocytes dedifferentiate during 2-dimensional (2D) expansion, MSCs are generally more studied and considered to have higher potential for cartilage repair purposes. Here we question if the dedifferentiated chondrocytes can regain the chondrogenic potential, to find potential applications in cartilage repair. For this we chose chondrocytes at passage 12 (considered to have sufficiently dedifferentiated) and the expression of chondrogenic phe- notypes and matrix syntheses were examined over 14 days. In particular, the chondrogenic potential of MSCs was also compared. Results showed that the dedifferentiated chondrocytes proliferated actively over 14 days with almost 2.5-fold increase relative to MSCs. Moreover, the chondrogenic ability of chondrocytes was significantly higher than that of MSCs, as confirmed by the expression of a series of mRNA levels and the production of cartilage extracellular matrix molecules in 2D-monolayer and 3-dimensional (3D)-spheroid cultures. Of note, the significance was higher in 3D-culture than in 2D- culture. Although more studies are needed such as the use of different cell passages and human cell source, and the chondrogenic confirmation under in vivo conditions, this study showing that the dedifferentiated chondrocytes can also be a suitable cell source for the cell-based cartilage repair, as a counterpart of MSCs, will encourage further studies regarding this issue.
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참고문헌 (57)
참고문헌 신청
Hunziker EB / 2002 / Articular cartilage repair: basic science and clinical progress. A review of the current status and prospects / Osteoarthritis Cartilage 10:432~463
Karuppal R / 2017 / Current concepts in the articular cartilage repair and regeneration / J Orthop 14:A1~A3
박도영 / 2016 / Repair of Partial Thickness Cartilage Defects Using Cartilage Extracellular Matrix Membrane-Based Chondrocyte Delivery System in Human Ex Vivo Model / 조직공학과 재생의학 13(2):182~190
장정호 / 2016 / Disc-Type Hyaline Cartilage Reconstruction Using 3D-Cell Sheet Culture of Human Bone Marrow Stromal Cells and Human Costal Chondrocytes and Maintenance of Its Shape and Phenotype after Transplantation / 조직공학과 재생의학 13(4):352~363
Yamashita A / 2010 / Identification of five developmental processes during chondrogenic differentiation of embryonic stem cells / PLoS One 5:e10998
Karuppal R / 2017 / Current concepts in the articular cartilage repair and regeneration / J Orthop 14:A1~A3
박도영 / 2016 / Repair of Partial Thickness Cartilage Defects Using Cartilage Extracellular Matrix Membrane-Based Chondrocyte Delivery System in Human Ex Vivo Model / 조직공학과 재생의학 13(2):182~190
장정호 / 2016 / Disc-Type Hyaline Cartilage Reconstruction Using 3D-Cell Sheet Culture of Human Bone Marrow Stromal Cells and Human Costal Chondrocytes and Maintenance of Its Shape and Phenotype after Transplantation / 조직공학과 재생의학 13(4):352~363
Yamashita A / 2010 / Identification of five developmental processes during chondrogenic differentiation of embryonic stem cells / PLoS One 5:e10998
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