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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 172 - 182 (11page)
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초록· 키워드
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Rheb (Ras homolog enriched in the brain) is a small GTPase protein that plays an important role in cell signaling for development of the neocortex through modulation of mTORC1 (mammalian-target-of-rapamycin-complex-1) activity. mTORC1 is known to control various biological processes including axonal growth in forming complexes at the lysosomal membrane compartment. As such, anchoring of Rheb on the lysosomal membrane via the farnesylation of Rheb at its cysteine residue (C180) is required for its promotion of mTOR activity. To test the significance of Rheb farnesylation, we overexpressed a farnesylation mutant form of Rheb, Rheb C180S, in primary rat hippocampal neurons and also in mouse embryonic neurons using in utero electroporation. Interestingly, we found that Rheb C180S maintained promotional effect of axonal elongation similar to the wild-type Rheb in both test systems. On the other hand, Rheb C180S failed to exhibit the multiple axon-promoting effect which is found in wild-type Rheb. The levels of phospho-4EBP1, a downstream target of mTORC1, were surprisingly increased in Rheb C180S transfected neurons, despite the levels of phosphorylated mTOR being significantly decreased compared to control vector transfectants. A specific mTORC1 inhibitor, rapamycin, also could not completely abolish axon elongation characteristics of Rheb C180S in transfected cells. Our data suggests that Rheb in a non-membrane compartment can promote the axonal elongation via phosphorylation of 4EBP1 and through an mTORC1-independent pathway.
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등록된 정보가 없습니다.
참고문헌 (44)
참고문헌 신청
Bradke F / 2000 / Establishment of neuronal polarity : lessons from cultured hippocampal neurons / Curr Opin Neurobiol 10:574~581
Blanquie O / 2018 / Cytoskeleton dynamics in axon regeneration / Curr Opin Neurobiol 51:60~69
Craig AM / 1994 / Neuronal polarity / Annu Rev Neurosci 17:267~310
da Silva JS / 2002 / Breaking the neuronal sphere : regulation of the actin cytoskeleton in neuritogenesis / Nat Rev Neurosci 3:694~704
Jaworski J / 2006 / The growing role of mTOR in neuronal development and plasticity / Mol Neurobiol 34:205~219
Blanquie O / 2018 / Cytoskeleton dynamics in axon regeneration / Curr Opin Neurobiol 51:60~69
Craig AM / 1994 / Neuronal polarity / Annu Rev Neurosci 17:267~310
da Silva JS / 2002 / Breaking the neuronal sphere : regulation of the actin cytoskeleton in neuritogenesis / Nat Rev Neurosci 3:694~704
Jaworski J / 2006 / The growing role of mTOR in neuronal development and plasticity / Mol Neurobiol 34:205~219
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