지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 396 - 404 (9page)
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Purpose: CD81 is a prognostic biomarker for high-grade bladder cancer (BC). In this study, we aimed to determine the functional mechanisms underlying the role of CD81 in BC progression. Materials and Methods: In two invasive BC cell lines (T24, J82), CD81 expression was suppressed by the transfection of lentiviral vectors including CD81-specific shRNAs, and then the migration and invasion of BC cells was analyzed. Enzymatic activity of matrix metalloproteinases (MMPs) was also analyzed by collagen-zymography. The expression of MMPs was confirmed by western blotting using culture supernatants from each cell line. Signaling pathways related to MMPs were investigated using various antibodies. Results: CD81 was successfully knocked down by shRNAs in T24 and J82 cell lines. While the migration of BC cells was not affected after the knockdown of CD81, the invasive activity was significantly increased in both cell lines. Zymography produced distinct bands using supernatants from CD81-knockdown cells, whereas only faint bands were observed with empty vector-transfected cells. We also observed an increased expression of MMPs, specifically MMP2 and 9, in the conditioned media from CD81-knockdown cells by western blotting. Mechanistically, the phosphorylation of extracellular signal-regulated kinase (ERK) was associated with the invasive activity of BC cells, while U0126 (an ERK inhibitor) reduced the invasive activity of CD81-knockdown BC cells. Conclusions: Taken together, CD81 suppression promotes the invasive property of BC cells through MMP signaling via ERK phosphorylation. Our results suggest that the regulation of CD81 expression may have some therapeutic potential in BC.
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참고문헌 (27)
참고문헌 신청
Global Burden of Disease Cancer Collaboration / 2017 / Global, regional, and national cancer incidence, mortality, years of life lost, years lived with disability, and disability-adjusted life-years for 32 cancer groups, 1990 to 2015: a systematic analysis for the Global Burden of Disease Study / JAMA Oncol 3:524~548
van Rhijn BW / 2009 / Recurrence and progression of disease in nonmuscle-invasive bladder cancer : from epidemiology to treatment strategy / Eur Urol 56:430~442
Makito Miyake / 2016 / Active surveillance for nonmuscle invasive bladder cancer / Investigative and Clinical Urology 57:4~13
Hall MC / 2007 / Guideline for the management of nonmuscle invasive bladder cancer(stages Ta, T1, and Tis) : 2007 update / J Urol 178:2314~2330
Babjuk M / 2008 / EAU guidelines on non-muscle-invasive urothelial carcinoma of the bladder / Eur Urol 54:303~314
van Rhijn BW / 2009 / Recurrence and progression of disease in nonmuscle-invasive bladder cancer : from epidemiology to treatment strategy / Eur Urol 56:430~442
Makito Miyake / 2016 / Active surveillance for nonmuscle invasive bladder cancer / Investigative and Clinical Urology 57:4~13
Hall MC / 2007 / Guideline for the management of nonmuscle invasive bladder cancer(stages Ta, T1, and Tis) : 2007 update / J Urol 178:2314~2330
Babjuk M / 2008 / EAU guidelines on non-muscle-invasive urothelial carcinoma of the bladder / Eur Urol 54:303~314
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