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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 856 - 869 (14page)
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To elucidate the mechanism of action of HOXA11-AS in modulating the cisplatin resistance of nasopharyngeal carcinoma (NPC) cells. HOXA11-AS and miR-454-3p expression in NPC tissue and cisplatin-resistant NPC cells were measured via quantitative reverse transcriptase polymerase chain reaction. NPC parental cells (C666-1 and HNE1) and cisplatin-resistant cells (C666-1/DDP and HNE1/DDP) were transfected and divided into different groups, after which the MTT method was used to determine the inhibitory concentration 50 (IC50) of cells treated with different concentrations of cisplatin. Additionally, a clone formation assay, flow cytometry and Western blotting were used to detect DDP-induced changes. Thereafter, xenograft mouse models were constructed to verify the in vitro results. Obviously elevated HOXA11-AS and reduced miR-454-3p were found in NPC tissue and cisplatin-resistant NPC cells. Compared to the control cells, cells in the si-HOXA11-AS group showed sharp decreases in cell viability and IC50, and these results were reversed in the miR-454-3p inhibitor group. Furthermore, HOXA11-AS targeted miR-454-3p, which further targeted c-Met. In comparison with cells in the control group, HNE1/DDP and C666-1/DDP cells in the si-HOXA11-AS group demonstrated fewer colonies, with an increase in the apoptotic rate, while the expression levels of c-Met, p-Akt/Akt and p-mTOR/mTOR decreased. Moreover, the si-HOXA11-AS-induced enhancement in sensitivity to cisplatin was abolished by miR-454-3p inhibitor transfection. The in vivo experiment showed that DDP in combination with si-HOXA11-AS treatment could inhibit the growth of xenograft tumors. Silencing HOXA11-AS can inhibit the c-Met/AKT/mTOR pathway by specifically upregulating miR-454-3p, thus promoting cell apoptosis and enhancing the sensitivity of cisplatin-resistant NPC cells to cisplatin.
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참고문헌 (36)
참고문헌 신청
Bao, X. / 2017 / Knockdown of long non-coding RNA HOTAIR increases miR-454-3p by targeting Stat3 and Atg12 to inhibit chondrosarcoma growth / Cell Death Dis 8:e2605
Bayne, K / 1996 / Revised guide for the care and use of laboratory animals available / American Physiological Society. Physiologist 39:199
Chen, Q. Y. / 2011 / Concurrent chemoradiotherapy vs radiotherapy alone in stage II nasopharyngeal carcinoma : phase III randomized trial / J. Natl. Cancer Inst 103:1761~1770
Colaco, R. J. / 2013 / Nasopharyngeal carcinoma: a retrospective review of demographics, treatment and patient outcome in a single centre / Clin. Oncol. (R. Coll. Radiol.) 25:171~177
Dai, C. / 2018 / MiR-206 inhibits epithelial ovarian cancer cells growth and invasion via blocking c-Met/AKT/mTOR signaling pathway / Biomed. Pharmacother 104:763~770
Bayne, K / 1996 / Revised guide for the care and use of laboratory animals available / American Physiological Society. Physiologist 39:199
Chen, Q. Y. / 2011 / Concurrent chemoradiotherapy vs radiotherapy alone in stage II nasopharyngeal carcinoma : phase III randomized trial / J. Natl. Cancer Inst 103:1761~1770
Colaco, R. J. / 2013 / Nasopharyngeal carcinoma: a retrospective review of demographics, treatment and patient outcome in a single centre / Clin. Oncol. (R. Coll. Radiol.) 25:171~177
Dai, C. / 2018 / MiR-206 inhibits epithelial ovarian cancer cells growth and invasion via blocking c-Met/AKT/mTOR signaling pathway / Biomed. Pharmacother 104:763~770
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