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논문 기본 정보

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학술저널
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한국생체재료학회 생체재료학회지 생체재료학회지 제23권 제4호
발행연도
2019.1
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147 - 159 (13page)

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Background: Collagen-based scaffolds reinforced with hydroxyapatite (HA) are an attractive choice for bone tissue engineering because their composition mimics that of bone. We previously reported the development of compression-molded collagen-HA scaffolds that exhibited high porosity, interconnected pores, and mechanical properties that were well-suited for surgical handling and fixation. The objective of this study was to investigate these novel collagen-HA scaffolds in combination with human adipose-derived stem cells (hASCs) as a template for bone formation in a subcutaneous athymic mouse model. Methods: Collagen-HA scaffolds and collagen-only scaffolds were fabricated as previously described, and a clinically approved bone void filler was used as a control for the material. Constructs were seeded with hASCs and were pretreated with either control or osteogenic media. A cell-free group was also included. Scaffolds were implanted subcutaneously in the backs of athymic nude mice for 8 weeks. Mineral depositi on was quantified via microcomputed tomography. Histological and immunofluorescence images of the explants were used to analyze their vascular invasion, remodeling and cellularity. Results: Cell-free collagen-HA scaffolds and those that were pre-seeded with osteogenically differentiated hASCs supported mineral deposition and vascular invasion at comparable rates, while cell-seeded constructs treated with the control medium showed lower mineralization after implantation. HA-reinforcement allowed collagen constructs to maintain their shape, provided improved cell-tissue-scaffold integration, and resulted in a more organized tissue when pre-treated in an osteogenic medium. Scaffold type and pre-treatment also determined osteoclast activity and therefore potential remodeling of the constructs. Conclusions: The results of this study cumulatively indicate that treatment medium and scaffold composition direct mineralization and angiogenic tissue formation in an ectopic model. The data suggest that it may be necessary to match the scaffold with a particular cell type and cell-specific pre-treatment to achieve optimal bone formation.

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참고문헌 (34)

참고문헌 신청
Komatsu DE / 2010 / The control of fracture healing and its therapeutic targeting : improving upon nature / J Cell Biochem 109:302~311 google schola Giannoudis PV / 2005 / Bone substitutes: an update / Injury 36(Supplement 3):S20~S27 google schola Burg KJLL / 2000 / Biomaterial developments for bone tissue engineering / Biomaterials 21:2347~2359 google schola Gleeson JP / 2010 / Addition of hydroxyapatite improves stiffness, interconnectivity and osteogenic potential of a highly porous collagen-based scaffold for bone tissue regeneration / Eur Cell Mater 20:218~230 google schola Kane RJ / 2012 / Effects of hydroxyapatite reinforcement on the architecture and mechanical properties of freeze-dried collagen scaffolds / J Mech Behav Biomed Mater 7:41~49 google schola

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