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논문 기본 정보

자료유형
학술저널
저자정보
Park, Hannara (Department of Plastic and Reconstructive Surgery, Daegu Fatima Hospital) Kim, Jin Soo (Department of Plastic and Reconstructive Surgery, Daegu Fatima Hospital) Oh, Eun Jung (Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook National University) Kim, Tae Jung (Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook National University) Kim, Hyun Mi (Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook National University) Shim, Jin Hyung (Department of Mechanical Engineering, Korea Polytechnic University) Yoon, Won Soo (Department of Mechanical Engineering, Korea Polytechnic University) Huh, Jung Bo (Department of Prosthodontics, Dental Research Institute, Institute of Translational Dental Science, School of Dentistry, Pusan National University) Moon, Sung Hwan (Department of Medicine, Konkuk University School of Medicine) Kang, Seong Soo (College of Veterinary Medicine, Chonnam National University) Chung, Ho Yun (Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook National University)
저널정보
대한두개안면성형외과학회 Archives of craniofacial surgery : ACFS Archives of craniofacial surgery : ACFS 제19권 제3호
발행연도
2018.1
수록면
181 - 189 (9page)

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Background: Autogenous bone grafts have several limitations including donor-site problems and insufficient bone volume. To address these limitations, research on bone regeneration is being conducted actively. In this study, we investigate the effects of a three-dimensionally (3D) printed polycaprolactone (PCL)/tricalcium phosphate (TCP) scaffold on the osteogenic differentiation potential of adipose tissue-derived stem cells (ADSCs) and bone marrow-derived stem cells (BMSCs). Methods: We investigated the extent of osteogenic differentiation on the first and tenth day and fourth week after cell culture. Cytotoxicity of the 3D printed $PCL/{\beta}-TCP$ scaffold was evaluated by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay, prior to osteogenic differentiation analysis. ADSCs and BMSCs were divided into three groups: C, only cultured cells; M, cells cultured in the 3D printed $PCL/{\beta}-TCP$ scaffold; D, cells cultured in the 3D printed $PCL/{\beta}-TCP$ scaffold with a bone differentiation medium. Alkaline phosphatase (ALP) activity assay, von Kossa staining, reverse transcription-polymerase chain reaction (RT-PCR), and Western blotting were performed for comparative analysis. Results: ALP assay and von Kossa staining revealed that group M had higher levels of osteogenic differentiation compared to group C. RT-PCR showed that gene expression was higher in group M than in group C, indicating that, compared to group C, osteogenic differentiation was more extensive in group M. Expression levels of proteins involved in ossification were higher in group M, as per the Western blotting results. Conclusion: Osteogenic differentiation was increased in mesenchymal stromal cells (MSCs) cultured in the 3D printed PCL/TCP scaffold compared to the control group. Osteogenic differentiation activity of MSCs cultured in the 3D printed PCL/TCP scaffold was lower than that of cells cultured on the scaffold in bone differentiation medium. Collectively, these results indicate that the 3D printed PCL/TCP scaffold promoted osteogenic differentiation of MSCs and may be widely used for bone tissue engineering.
#Polycaprolactone #Tricalcium phosphate #Adipose tissue #Bone marrow #Stem cells #Cell differentiation #Mesenchymal stromal cells #Tissue engineering

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