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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 1 - 17 (17page)
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Three-dimensional microscopic approaches in histopathology display multiplex properties that present puzzling questions for specimens as related to their comprehensive volumetric information. This information includes spatial distribution of molecules, three-dimensional co-localization, structural formation and whole data set that cannot be determined by two-dimensional section slides due to the inevitable loss of spatial information. Advancement of optical instruments such as two-photon microscopy and high performance objectives with motorized correction collars have narrowed the gap between optical theories and the actual reality of deep tissue imaging. However, the benefits gained by a prolonged working distance, two-photon laser and optimized beam alignment are inevitably diminished because of the light scattering phenomenon that is deeply related to the refractive index mismatch between each cellular component and the surrounding medium. From the first approaches with simple crude refractive index matching techniques to the recent cutting-edge integrated tissue clearing methods, an achievement of transparency without morphological denaturation and eradication of natural and fixation-induced nonspecific autofluorescence out of real signal are key factors to determine the perfection of tissue clearing and the immunofluorescent staining for high contrast images. When performing integrated laboratory workflow of tissue for processing frozen and formalin-fixed tissues, clear lipid-exchanged acrylamide-hybridized rigid imaging/immunostaining/in situ hybridization-compatible tissue hydrogel (CLARITY), an equipment-based tissue clearing method, is compatible with routine procedures in a histopathology laboratory.
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참고문헌 (82)
참고문헌 신청
Viviana Gradinaru / 2018 / Hydrogel-Tissue Chemistry: Principles and Applications / Annual Review of Biophysics 47(1):355~376
Anna Khimchenko / 2016 / Extending two-dimensional histology into the third dimension through conventional micro computed tomography / NeuroImage 139:26~36
P.A. YOUNG / 2011 / The effects of refractive index heterogeneity within kidney tissue on multiphoton fluorescence excitation microscopy / Journal of Microscopy 242(2):148~156
Daniel L. Marks / 2008 / Plastinated tissue samples as three-dimensional models for optical instrument characterization / Optics Express 16(20):16272~16283
Yong Woong Jun / 2017 / Addressing the autofluorescence issue in deep tissue imaging by two-photon microscopy: the significance of far-red emitting dyes / Chem. Sci. 8(11):7696~7704
Anna Khimchenko / 2016 / Extending two-dimensional histology into the third dimension through conventional micro computed tomography / NeuroImage 139:26~36
P.A. YOUNG / 2011 / The effects of refractive index heterogeneity within kidney tissue on multiphoton fluorescence excitation microscopy / Journal of Microscopy 242(2):148~156
Daniel L. Marks / 2008 / Plastinated tissue samples as three-dimensional models for optical instrument characterization / Optics Express 16(20):16272~16283
Yong Woong Jun / 2017 / Addressing the autofluorescence issue in deep tissue imaging by two-photon microscopy: the significance of far-red emitting dyes / Chem. Sci. 8(11):7696~7704
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