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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
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- 1 - 10 (10page)
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Purpose: Diquafosol is a pharmaceutical drug used for dry eye treatment with a novel mechanism of action. It is a purinergic P2Y2 receptor agonist that promotes the secretion of tears and healing of corneal epithelialwounds. However, its inhibitory effect on hyperosmotic stress-induced inflammation in human corneal epithelialcells (HCECs) remains unclear. Methods: A hyperosmotic stress model was established by transferring HCECs from isosmotic (312 mOsm/kg tohyperosmotic medium (500 mOsm/kg). HCECs were incubated with 500 mOsm/kg hyperosmotic medium for30 minutes, and then treated with diquafosol (0.6–6 mg/mL) for 4 or 24 hours. Cells were then harvested andanalyzed by western blot, immunocytochemistry, and real-time polymerase chain reaction to evaluate the expressionof interleukin-6, tumor necrosis factor-alpha, and the phosphorylation status of nuclear factor-kappa B. Results: Diquafosol significantly decreased the mRNA and protein expression of hyperosmotic stress-inducedtumor necrosis factor-alpha and interleukin-6. These results were supported by immunofluorescence stainingand quantitative real-time polymerase chain reaction analysis. Furthermore, diquafosol inhibits nuclear factor-kappa B activation by suppressing the phosphorylation and degradation of the inhibitor of кB. Conclusions: This study shows that diquafosol inhibits nuclear factor-kappa B signaling and inflammatory factorsinduced by hyperosmotic stress in HCECs. This suggests that using diquafosol for the improvement of dryeye syndrome could be effective in the treatment of inflammation-related corneal and conjunctival diseases.
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참고문헌 (30)
참고문헌 신청
Gulati S / 2017 / Ocular pharmacology of tear film, dry eye, and allergic conjunctivitis / Handb Exp Pharmacol 242:97~118
McCarty CA / 1998 / The epidemiology of dry eye in Melbourne, Australia / Ophthalmology 105:1114~1119
Kuang TM / 2007 / Correctable visual impairment in an elderly Chinese population in Taiwan : the Shihpai Eye Study / Invest Ophthalmol Vis Sci 48:1032~1037
Miljanovic B / 2007 / Impact of dry eye syndrome on vision-related quality of life / Am J Ophthalmol 143:409~415
Versura P / 2011 / Hyperosmolar stress upregulates HLA-DR expression in human conjunctival epithelium in dry eye patients and in vitro models / Invest Ophthalmol Vis Sci 52:5488~5496
McCarty CA / 1998 / The epidemiology of dry eye in Melbourne, Australia / Ophthalmology 105:1114~1119
Kuang TM / 2007 / Correctable visual impairment in an elderly Chinese population in Taiwan : the Shihpai Eye Study / Invest Ophthalmol Vis Sci 48:1032~1037
Miljanovic B / 2007 / Impact of dry eye syndrome on vision-related quality of life / Am J Ophthalmol 143:409~415
Versura P / 2011 / Hyperosmolar stress upregulates HLA-DR expression in human conjunctival epithelium in dry eye patients and in vitro models / Invest Ophthalmol Vis Sci 52:5488~5496
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