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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한약리학회 The Korean Journal of Physiology & Pharmacology The Korean Journal of Physiology & Pharmacology 제21권 제2호
- 발행연도
- 2017.3
- 수록면
- 189 - 195 (7page)
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This study aimed to compare the cellular toxicities of three clinically used dry eye treatments; 3% diquafosol tetrasodium and hyaluronic acid at 0.3 and 0.18%. A methyl thiazolyltetrazoiun (MTT)-based calorimetric assay was used to assess cellular proliferation and a lactate dehydrogenase (LDH) leakage assay to assess cytotoxicity, using Human corneal epithelial cells (HCECs) exposed to 3% diquafosol tetrasodium, 0.3% hyaluronic acid (HA), or 0.18% HA or 1, 6 or 24 h.
Cellular morphology was evaluated by inverted phase-contrast light microscopy and electron microscopy, and wound widths were measured 24 h after confluent HCECs were scratched. Diquafosol had a significant, time-dependent, inhibitory effect on HCEC proliferation and cytotoxicity. HCECs treated with diquafosol detached more from the bottoms of dishes and damaged cells showed degenerative changes, such as, reduced numbers of microvilli, vacuole formation, and chromatin of the nuclear remnant condensed along the nuclear periphery. All significantly stimulated reepithelialization of HCECs scratched, which were less observed in diquafosol. Therefore, epithelial toxicity should be considered after long-term usage of diquafosol and in overdose cases, especially in dry eye patients with pre-existing punctated epithelial erosion.
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참고문헌 (26)
참고문헌 신청
Uchino M / 2011 / Prevalence and risk factors of dry eye disease in Japan : Koumi study / Ophthalmology 118:2361~2367
Lee JE / 2014 / A randomised controlled trial comparing a thermal massager with artificial teardrops for the treatment of dry eye / Br J Ophthalmol 98:46~51
Gomes JA / 2004 / Sodium hyaluronate(hyaluronic acid)promotes migration of human corneal epithelial cells in vitro / Br J Ophthalmol 88:821~825
Yamaguchi M / 2012 / The safety and efficacy of long-term treatment with 3% diquafosol ophthalmic solution for dry eye / Atarashii Ganka(J Eye) 29:527~535
Takaoka-Shichijo Y / 2011 / Effect of diquafosol tetrasodium on MUC5AC secretion by rabbit conjunctival tissues / Atarashii Ganka(J Eye) 28:261~265
Lee JE / 2014 / A randomised controlled trial comparing a thermal massager with artificial teardrops for the treatment of dry eye / Br J Ophthalmol 98:46~51
Gomes JA / 2004 / Sodium hyaluronate(hyaluronic acid)promotes migration of human corneal epithelial cells in vitro / Br J Ophthalmol 88:821~825
Yamaguchi M / 2012 / The safety and efficacy of long-term treatment with 3% diquafosol ophthalmic solution for dry eye / Atarashii Ganka(J Eye) 29:527~535
Takaoka-Shichijo Y / 2011 / Effect of diquafosol tetrasodium on MUC5AC secretion by rabbit conjunctival tissues / Atarashii Ganka(J Eye) 28:261~265
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