지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제2호
- 발행연도
- 2015.1
- 수록면
- 274 - 279 (6page)
이용수
초록· 키워드
오류제보하기
Receptor activator of nuclear factor-kappa B ligand (RANKL) is a critical factor in osteoclastogenesis. It makes osteoclasts differentiate and multinucleate in bone remodeling. In the present study, RANKL was expressed as a soluble maltose binding protein (MBP)-fusion protein using the Escherichia coli maltose binding domain tag system (pMAL) expression vector system. The host cell E. coli DH5α was cultured and induced by isopropyl β-D-1- thiogalactopyranoside for rRANKL expression. Cells were disrupted by sonication to collect soluble MBP-fused rRANKL. The MBP-fusion rRANKL was purified with MBP Trap affinity chromatography and treated with Tobacco Etch Virus nuclear inclusion endopeptidase (TEV protease) to remove the MBP fusion protein. Dialysis was then carried out to remove binding maltose from the cleaved rRANKL solution. The cleaved rRANKL was purified with a second MBP Trap affinity chromatography to separate unsevered MBP-fusion rRANKL and cleaved MBP fusion protein. The purified rRANKL was shown to have biological activity by performing in vitro cell tests. In conclusion, biologically active rRANKL was successfully purified by a simple two-step chromatography purification process with one column.
목차
등록된 정보가 없습니다.
참고문헌 (16)
참고문헌 신청
Anderson DM / 1997 / A homologue of the TNF receptor and its ligand enhance T-cell growth and dendritic-cell function / Nature 390 : 175 ~ 179
Boyce BF / 2008 / Functions of RANKL/RANK/OPG in bone modeling and remodeling / Arch. Biochem. Biophys 473 : 139 ~ 146
Fata JE / 2000 / The osteoclast differentiation factor osteoprotegerin-ligand is essential for mammary gland development / Cell 103 : 41 ~ 50
Gallagher JC / 1994 / Diagnosis, prophylaxis, and treatment of osteoporosis / Am. J. Med 90 : 646 ~ 650
Grigoriades AE / 1994 / c-Fos: a key regulator of osteoclast-macrophage lineage determination and bone remodeling / Science 266 : 443 ~ 448
Boyce BF / 2008 / Functions of RANKL/RANK/OPG in bone modeling and remodeling / Arch. Biochem. Biophys 473 : 139 ~ 146
Fata JE / 2000 / The osteoclast differentiation factor osteoprotegerin-ligand is essential for mammary gland development / Cell 103 : 41 ~ 50
Gallagher JC / 1994 / Diagnosis, prophylaxis, and treatment of osteoporosis / Am. J. Med 90 : 646 ~ 650
Grigoriades AE / 1994 / c-Fos: a key regulator of osteoclast-macrophage lineage determination and bone remodeling / Science 266 : 443 ~ 448
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
MBP fusion proteins: enhanced expression and solubility, unexpected cleavage dilemma
Biodesign
2024 .09
-
Soluble Expression of Artificially Designed Recombinant Protein through Conjugating Fusion Protein in Escherichia coli
한국미생물학회 학술대회논문집
2017 .04
-
Effects of Co-Expression of Liver X Receptor β-Ligand Binding Domain with its Partner, Retinoid X Receptor α-Ligand Binding Domain, on their Solubility and Biological Activity in Escherichia coli
Journal of Microbiology and Biotechnology
2015 .01
-
Design of a RANK-Mimetic Peptide Inhibitor of Osteoclastogenesis with Enhanced RANKL-Binding Affinity
Molecules and Cells
2016 .04
-
Rapid purification of HRV3C and TEV proteases using polyhistidine and polylysine tags
Biodesign
2020 .01
-
The Inactivation of ERK1/2, p38 and NF-kB Is Involved in the Down-Regulation of Osteoclastogenesis and Function by A2B Adenosine Receptor Stimulation
Molecules and Cells
2017 .10
-
Engineering of Recombinant Human Papillomavirus 16 L1 Protein for Incorporation with para -Azido- L -Phenylalanine
Journal of Microbiology and Biotechnology
2024 .09
-
Caspase-3-facilitated Stoichiometric Cleavage of a Large Recombinant Polyprotein
생명과학회지
2015 .04
-
Codon Optimization, Soluble Expression and Purification of PE_PGRS45 Gene from Mycobacterium tuberculosis and Preparation of Its Polyclonal Antibody Protein
Journal of Microbiology and Biotechnology
2021 .11
-
Interacting Proteins in Salmonella enterica Can be Purified with Sequential Peptide Affinity Purification System
한국미생물학회 학술대회논문집
2022 .10
-
MicroRNA-26a Regulates RANKL-Induced Osteoclast Formation
Molecules and Cells
2015 .01
-
Production of Biopharmaceuticals in E. coli: Current Scenario and Future Perspectives
Journal of Microbiology and Biotechnology
2015 .01
-
[FUSION POWER] 융합의 새로운 시대
BBC 사이언스
2022 .09
-
Antimicrobial agents, outer membrane assembly machine, lipoprotein, lipopolysaccharide, outer membrane β-barrel protein, phospholipid
Journal of Microbiology and Biotechnology
2019 .01
-
Immune Modulation of Recombinant OmpA against Brucella abortus 544 Infection in Mice
Journal of Microbiology and Biotechnology
2016 .01
-
PARK2 Induces Osteoclastogenesis through Activation of the NF-κB Pathway
Molecules and Cells
2022 .10
-
Cell Fusion and its Therapeutic Development
한국분석과학회 학술대회
2022 .05
-
Current Understanding of RANK Signaling in Osteoclast Differentiation and Maturation
Molecules and Cells
2017 .10
-
Construction, Investigation and Application of TEV Protease Variants with Improved Oxidative Stability
Journal of Microbiology and Biotechnology
2021 .12
-
Purification, Characterization, and Cloning of a Cold-Adapted Protease from Antarctic Janthinobacterium lividum
Journal of Microbiology and Biotechnology
2018 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0