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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제3호
- 발행연도
- 2018.1
- 수록면
- 448 - 453 (6page)
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In this study, a 107 kDa protease from psychrophilic Janthinobacterium lividum PAMC 26541 was purified by anion-exchange chromatography. The specific activity of the purified protease was 264 U/mg, and the overall yield was 12.5%. The J. lividum PAMC 25641 protease showed optimal activity at pH 7.0–7.5 and 40oC. Protease activity was inhibited by PMSF, but not by DTT. On the basis of the N-terminal sequence of the purified protease, the gene encoding the cold-adapted protease from J. lividum PAMC 25641 was cloned into the pET-28a(+) vector and heterologously expressed in Escherichia coli BL21(DE3) as an intracellular soluble protein.
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참고문헌 (21)
참고문헌 신청
Margesin R / 2010 / Biotechnological applications of psychrophiles / Environ. Technol. 31:835~844
Javed A / 2016 / Psychrophilic microbial enzymes implications in coming biotechnological processes / Am. Sci. Res. J. Eng. Technol. Sci. 23:103~120
Mienda B S / 2014 / An overview of microbial proteases for industrial applications / Res. J. Pharm. Biol. Chem. Sci. 5:388~396
Vazquez SC / 2004 / Extracellular proteases from eight psychrotolerant Antarctic strains / Microbiol. Res. 159:157~166
Joshi S / 2013 / Biotechnology of cold-active proteases / Biology 2:755~783
Javed A / 2016 / Psychrophilic microbial enzymes implications in coming biotechnological processes / Am. Sci. Res. J. Eng. Technol. Sci. 23:103~120
Mienda B S / 2014 / An overview of microbial proteases for industrial applications / Res. J. Pharm. Biol. Chem. Sci. 5:388~396
Vazquez SC / 2004 / Extracellular proteases from eight psychrotolerant Antarctic strains / Microbiol. Res. 159:157~166
Joshi S / 2013 / Biotechnology of cold-active proteases / Biology 2:755~783
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