지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제10호
- 발행연도
- 2010.1
- 수록면
- 1,378 - 1,385 (8page)
이용수
초록· 키워드
오류제보하기
A novel agarolytic bacterium, KY-YJ-3, producing extracellular agarase, was isolated from the freshwater sediment of the Sincheon River in Daegu, Korea. On the basis of Gramstaining data, morphology, and phylogenetic analysis of the 16S rDNA sequence, the isolate was identified as Cellvibrio sp. By ammonium sulfate precipitation followed by Toyopearl QAE-550C, Toyopearl HW-55F, and Mono-Q column chromatographies, the extracellular agarase in the culture fluid could be purified 120.2-fold with a yield of 8.1%. The specific activity of the purified agarase was 84.2 U/mg. The molecular mass of the purified agarase was 70 kDa as determined by dodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE). The optimal temperature and pH of the purified agarase were 35oC and pH 7.0, respectively. The purified agarase failed to hydrolyze the other polysaccharide substrates, including carboxymethyl-cellulose, dextran, soluble starch, pectin,and polygalacturonic acid. Kinetic analysis of the agarose hydrolysis catalyzed by the purified agarase using thinlayer chromatography showed that the main products were neoagarobiose, neoagarotetraose, and neoagarohexaose.
These results demonstrated that the newly isolated freshwater agarolytic bacterium KY-YJ-3 was a Cellvibrio sp., and could produce an extracellular β-agarase, which hydrolyzed agarose to yield neoagarobiose, neoagarotetraose,and neoagarohexaose as the main products.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Allouch, J / 2003 / The three-dimensional structures of two β-agarases / J. Biol. Chem 278 : 47171 ~ 47180
Aoki, T / 1990 / Purification and characterization of a novel β-agarase from Vibrio sp. AP-2 / Eur. J. Biochem 187 : 461 ~ 465
Bannikova, G. E / 2008 / The thermophilic bacteria hydrolyzing agar: Characterization of thermostable agarase / Appl. Biochem. Microbiol 45 : 366 ~ 371
Bradford, M / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding / Anal. Biochem 72 :
Buttner, M. J / 1987 / The agarase gene (dagA) of Streptomyces coelicolor (A3)2: Nucleotide sequence and transcriptional analysis / Mol. Gen. Genet 209 : 101 ~ 109
Aoki, T / 1990 / Purification and characterization of a novel β-agarase from Vibrio sp. AP-2 / Eur. J. Biochem 187 : 461 ~ 465
Bannikova, G. E / 2008 / The thermophilic bacteria hydrolyzing agar: Characterization of thermostable agarase / Appl. Biochem. Microbiol 45 : 366 ~ 371
Bradford, M / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding / Anal. Biochem 72 :
Buttner, M. J / 1987 / The agarase gene (dagA) of Streptomyces coelicolor (A3)2: Nucleotide sequence and transcriptional analysis / Mol. Gen. Genet 209 : 101 ~ 109
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Cloning of Agarase Gene from Non-Marine Agarolytic Bacterium Cellvibrio sp.
Journal of Microbiology and Biotechnology
2012 .01
-
Agarase의 분류, 기원, 확보, 활성파악, 분리정제, 생산 및 응용
생명과학회지
2012 .02
-
한천분해효소의 재조합발현 : 기원, 활성조건, 분비신호와 게놈분석 등
생명과학회지
2020 .03
-
Purification and Characterization of Thermostable Agarase from Bacillus sp. BI-3, a Thermophilic Bacterium Isolated from Hot Spring
Journal of Microbiology and Biotechnology
2014 .01
-
Purification and Characterization of Agarase from Agarolytic strain AS-1
한국생명과학회 심포지움
2006 .04
-
해양세균 Agarivorans sp. BK-1의 분리 및 β-아가라제의 특성 규명
생명과학회지
2019 .11
-
서해안 해수로부터 분리한 한천분해 해양미생물 Pseudoalteromonas sp. H9의 동정 및 특성 연구
한국미생물·생명공학회지
2015 .01
-
제주도 연안 해양에서 분리한 한천분해 미생물 Vibrio sp. S4의 동정 및 내열성 agarase의 생화학적 특성
한국미생물·생명공학회지
2015 .01
-
재조합 한천 분해효소의 생산과 응용
한국해양바이오학회지
2016 .06
-
Cloning, Expression and Characterization of β-agarase Gene from a Marine Bacterium, Pseudoalteromonas sp. JT-6
생명과학회지
2008 .05
-
Cloning, Expression, and Characterization of a Novel GH-16 β-Agarase from Agarivorans sp. JA-1
생명과학회지
2012 .11
-
해양성 Simiduia sp. SH-1 균주의 분리 및 한천분해효소의 특성조사
생명과학회지
2015 .11
-
Gene Cloning, Expression, and Characterization of a β-Agarase, AgaB34, from Agarivorans albus YKW-34
Journal of Microbiology and Biotechnology
2009 .01
-
Purification and Characterization of Agarase from Agarolytic strain Sphingomonas paucimobilis
한국생명과학회 심포지움
2006 .09
-
해양미생물 Sphingomonas paucimobilis AS-1이 생산하는 새로운 extracelluar agarase의 정제 및 특성
생명과학회지
2008 .01
-
Cloning, Expression, and Characterization of a Glycoside Hydrolase Family 118 β-Agarase from Agarivorans sp. JA-1
Journal of Microbiology and Biotechnology
2012 .01
-
Molecular Cloning, Overexpression, and Enzymatic Characterization of Glycosyl Hydrolase Family 16 β-Agarase from Marine Bacterium Saccharophagus sp. AG21 in Escherichia coli
Journal of Microbiology and Biotechnology
2013 .01
-
Isolation and Characterization of a Novel Agarase-Producing Pseudoalteromonas spp. Bacterium from the Guts of Spiny Turban Shells
Journal of Microbiology and Biotechnology
2011 .01
-
Properties of agarase from a noble marine bacterium SL-5
한국생명과학회 심포지움
2006 .09
-
Characterization of an extracellular β-agarase from marine bacterium Agarivorans sp. JA-1
한국생명과학회 심포지움
2005 .04
이 논문의 저자 정보
최근 본 자료
댓글(0)
0