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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제9호
- 발행연도
- 2012.1
- 수록면
- 1,237 - 1,244 (8page)
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Agarase genes of non-marine agarolytic bacterium Cellvibrio sp. were cloned into Escherichia coli and one of the genes obtained using HindIII was sequenced. From nucleotide and putative amino acid sequences (713 aa,molecular mass; 78,771 Da) of the gene, designated as agarase AgaA, the gene was found to have closest homology to the Saccharophagus degradans (formerly,Microbulbifer degradans) 2-40 aga86 gene, belonging to glycoside hydrolase family 86 (GH86). The putative protein appears to be a non-secreted protein because of the absence of a signal sequence. The recombinant protein was purified with anion exchange and gel filtration columns after ammonium sulfate precipitation and the molecular mass (79 kDa) determined by SDS-PAGE and subsequent enzymography agreed with the estimated value,suggesting that the enzyme is monomeric. The optimal pH and temperature for enzymatic hydrolysis of agarose were 6.5 and 42.5ºC, and the enzyme was stable under 40ºC.
LC-MS and NMR analyses revealed production of a neoagarobiose and a neoagarotetraose with a small amount of a neoagarohexaose during hydrolysis of agarose, indicating that the enzyme is a β-agarase.
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참고문헌 (22)
참고문헌 신청
Ajisaka, K. / 2009 / Evaluation and comparison of the antioxidative potency of various carbohydrates using different methods / J. Agric. Food Chem 57 : 3102 ~ 3107
Aoki, T. / 1990 / Purification and characterization of a novel β-agarase from Vibrio sp. AP-2 / Eur. J. Biochem 187 : 461 ~ 465
Araki, T. / 1998 / Purification and characterization of agarases from amarine bacterium, Vibrio sp. PO-303 / J. Marine Biotechnol 6 : 260 ~ 265
Birnboim, H. C. / 1979 / A rapid alkaline extraction procedure for screening recombinant plasmid DNA / Nucleic Acids Res 7 : 1513 ~ 1523
Buttner, M. J. / 1987 / The agarase gene (dagA) of Streptomyces coelicolor A3 (2): Nucleotide sequence and transcriptional analysis / Mol. Gen. Genet 209 : 101 ~ 109
Aoki, T. / 1990 / Purification and characterization of a novel β-agarase from Vibrio sp. AP-2 / Eur. J. Biochem 187 : 461 ~ 465
Araki, T. / 1998 / Purification and characterization of agarases from amarine bacterium, Vibrio sp. PO-303 / J. Marine Biotechnol 6 : 260 ~ 265
Birnboim, H. C. / 1979 / A rapid alkaline extraction procedure for screening recombinant plasmid DNA / Nucleic Acids Res 7 : 1513 ~ 1523
Buttner, M. J. / 1987 / The agarase gene (dagA) of Streptomyces coelicolor A3 (2): Nucleotide sequence and transcriptional analysis / Mol. Gen. Genet 209 : 101 ~ 109
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