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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제12호
- 발행연도
- 2012.1
- 수록면
- 1,692 - 1,697 (6page)
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We report a glycoside hydrolase (GH)-118 β-agarase from a strain of Agarivorans, in which we previously reported recombinant expression and characterization of the GH-50 β-agarase. The GH comprised an open reading frame of 1,437 base pairs, which encoded a protein of 52,580daltons consisting of 478 amino acid residues. Assessment of the entire sequence showed that the enzyme had 97%nucleotide and 99% amino acid sequence similarities to those of GH-118 β-agarase from Pseudoalteromonas sp.
CY24, which belongs to a different order within the same class. The gene corresponding to a mature protein of 440amino acids was inserted, recombinantly expressed in Escherichia coli, and purified to homogeneity with affinity chromatography. It had maximal activity at 35oC and pH 7.0 and had 208.1 units/mg in the presence of 300 mM NaCl and 1 mM CaCl2. More than 80% activity was maintained after 2 h exposure to 35oC; however, < 40%activity remained at 45oC. The enzyme hydrolyzed agarose to yield neoagarooctaose as the main product.
This enzyme could be useful for industrial production of functional neoagarooligosaccharides.
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참고문헌 (22)
참고문헌 신청
[학술지
Allouch, J. / 2003 / The three-dimensional structures of two β-agarases / J. Biol. Chem 278 : 47171 ~ 47180
Araki, T. / 1998 / Optimization of parameters for isolation of protoplasts from Gracilaria verrucosa (Rhodophyta) / J. Mar. Biotechnol 6 : 193 ~ 197
Belas, R. / 1988 / Cloning and gene replacement mutagenesis of a Pseudomonas atlantica agarase gene / Appl. Environ. Microbiol 54 : 30 ~ 37
Dong, J. / 2006 / Cloning of the novel gene encoding β-agarase C from amarine bacterium, Vibrio sp. strain PO-303, and characterizationof the gene product / Appl. Environ. M
Allouch, J. / 2003 / The three-dimensional structures of two β-agarases / J. Biol. Chem 278 : 47171 ~ 47180
Araki, T. / 1998 / Optimization of parameters for isolation of protoplasts from Gracilaria verrucosa (Rhodophyta) / J. Mar. Biotechnol 6 : 193 ~ 197
Belas, R. / 1988 / Cloning and gene replacement mutagenesis of a Pseudomonas atlantica agarase gene / Appl. Environ. Microbiol 54 : 30 ~ 37
Dong, J. / 2006 / Cloning of the novel gene encoding β-agarase C from amarine bacterium, Vibrio sp. strain PO-303, and characterizationof the gene product / Appl. Environ. M
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