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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제5호
- 발행연도
- 2008.1
- 수록면
- 933 - 941 (9page)
이용수
초록· 키워드
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An extracellular β-glucosidase produced by Monascus
purpureus NRRL1992 in submerged cultivation was purified
by acetone precipitation, gel filtration, and hydrophobic
interaction chromatography, resulting in a purification
factor of 92-fold. A 22 central-composite design (CCD) was
performed to find the best temperature and pH conditions
for enzyme activity. Maximum activity was observed in a
wide range of temperature and pH values, with optimal
conditions set at 50oC and pH 5.5. The β-glucosidase
showed moderate thermostability, was inhibited by HgCl2,
K2CrO4, and K2Cr2O7, whereas other reagents including β-
mercaptoethanol, SDS, and EDTA showed no effect. Activity
was slightly stimulated by low concentrations of ethanol and
methanol. Hydrolysis of p-nitrophenyl-β-D-glucopyranoside
(pNPG), cellobiose, salicin, n-octyl-β-D-glucopyranoside,
and maltose indicates that the β-glucosidase has broad
substrate specificity. Apparently, glucosyl residues were
removed from the nonreducing end of p-nitrophenyl-β-Dcellobiose.
β-Glucosidase affinity and hydrolytic efficiency
were higher for pNPG, followed by maltose and cellobiose.
Glucose and cellobiose competitively inhibited pNPG
hydrolysis.
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등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
Badhan, A. K. / 7099 / Production of multiple xylanolytic and cellulolytic enzymes by thermophilic fungus Myceliophthora sp. IMI 387099. Bioresour / Technol 98 : 504 ~ 510
Belancic, A. / 2003 / β- Glucosidase from the native yeast Debaryomyces vanrijiae: Purification, characterization, and its effect on monoterpene content of a Muscat grape ju
Bhatia, Y. / 2002 / Microbial β- glucosidases: Cloning, properties and applications. Crit. Rev / Biotechnol 22 : 375 ~ 407
Box, G. E. P. / 1978 / Statistics for Experiments / Wiley
Cervantes, C. / 2001 / Interactions of chromium with microorganisms and plants. FEMS Microbiol / Rev 25 : 335 ~ 347
Belancic, A. / 2003 / β- Glucosidase from the native yeast Debaryomyces vanrijiae: Purification, characterization, and its effect on monoterpene content of a Muscat grape ju
Bhatia, Y. / 2002 / Microbial β- glucosidases: Cloning, properties and applications. Crit. Rev / Biotechnol 22 : 375 ~ 407
Box, G. E. P. / 1978 / Statistics for Experiments / Wiley
Cervantes, C. / 2001 / Interactions of chromium with microorganisms and plants. FEMS Microbiol / Rev 25 : 335 ~ 347
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