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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제21권 제5호
- 발행연도
- 2011.1
- 수록면
- 503 - 508 (6page)
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초록· 키워드
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A β-glucosidase from Phoma sp. KCTC11825BP isolated from rotten mandarin peel was purified 8.5-fold with a specific activity of 84.5 U/mg protein. The purified enzyme had a molecular mass of 440 kDa with a subunit of 110 kDa. The partial amino acid sequence of the purified β-glucosidase evidenced high homology with the fungal β-glucosidases belonging to glycosyl hydrolase family 3. Its optimal activity was detected at pH 4.5 and 60oC, and the enzyme had a half-life of 53 h at 60oC. The Km values for p-nitrophenyl-β-D-glucopyranoside and cellobiose were 0.3 mM and 3.2 mM, respectively. The enzyme was competitively inhibited by both glucose (Ki=1.7 mM) and glucono-δ-lactone (Ki=0.1 mM) when pNPG was used as the substrate. Its activity was inhibited by 41% by 10 mM Cu2+ and stimulated by 20% by 10 mM Mg2+.
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참고문헌 (21)
참고문헌 신청
Bradford, M. M / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72
Conner, R. L / 2000 / Impact of a disease and a defoliating insect on houndstongue (Cynoglossum officinale) growth: Implications for weed biological control / Ann. Appl. Biol
Coughlan,M.P / 1992 / Enzymic hydrolysis of cellulose: An overview / Bioresour. Technol 39 : 107 ~ 115
Daroit, Daniel J / 2008 / Purification and Characterization of an Extracellular β-Glucosidase fromMonascus purpureus / Journal of Microbiology and Biotechnology 18 (5) : 933
Decker, C. H / 2000 / β-Glucosidases from five black Aspergillus species: Study of their physicochemical and biocatalytic properties / J. Agric. Food Chem 48 : 4929 ~ 4936
Conner, R. L / 2000 / Impact of a disease and a defoliating insect on houndstongue (Cynoglossum officinale) growth: Implications for weed biological control / Ann. Appl. Biol
Coughlan,M.P / 1992 / Enzymic hydrolysis of cellulose: An overview / Bioresour. Technol 39 : 107 ~ 115
Daroit, Daniel J / 2008 / Purification and Characterization of an Extracellular β-Glucosidase fromMonascus purpureus / Journal of Microbiology and Biotechnology 18 (5) : 933
Decker, C. H / 2000 / β-Glucosidases from five black Aspergillus species: Study of their physicochemical and biocatalytic properties / J. Agric. Food Chem 48 : 4929 ~ 4936
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