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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제7호
- 발행연도
- 2016.1
- 수록면
- 1,206 - 1,215 (10page)
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초록· 키워드
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Ginsenosides are the major active ingredients in ginseng used for human therapeutic plant medicines. One of the most well-known probiotic bacteria among the various strains on the functional food market is Lactobacillus rhamnosus GG. Biocatalytic methods using probiotic enzymes for producing deglycosylated ginsenosides such as Rd have a growing significance in the functional food industry. The addition of 2% cellobiose (w/v) to glucose-free de Man-Rogosa-Sharpe broths notably induced β-glucosidase production from L. rhamnosus GG. Enzyme production and activity were optimized at a pH, temperature, and cellobiose concentration of 6.0, 40°C, and 2% (w/v), respectively. Under these controlled conditions, β-glucosidase production in L. rhamnosus GG was enhanced by 25-fold. Additionally, wholecell homogenates showed the highest β-glucosidase activity when compared with disrupted cell suspensions; the cell disruption step significantly decreased the β-glucosidase activity. Based on the optimized enzyme conditions, whole-cell L. rhamnosus GG was successfully used to convert ginsenoside Rb1 into Rd.
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참고문헌 (57)
참고문헌 신청
Adachi N / 2013 / Direct L -lysine production from cellobiose by Corynebacterium glutamicum displaying betaglucosidase on its cell surface. Appl / Microbiol. Biotechnol 97:7165~7172
Adsul M / 2007 / Production of lactic acid from cellobiose and cellotriose by Lactobacillus delbrueckii mutant Uc-3 / Appl. Environ. Microbiol 73:5055~5057
Atkinson AJ / 2007 / Principles of Clinical Pharmacology / Elsevier Inc :18~20
Balejko E / 2012 / Influence of Lactobacillus rhamnosus gg methabolites on growth of periodontal diseases bacteria / Electron. J. Polish Agric. Univ 15:1~7
Berdanier CD / 2007 / Handbook of Nutrition and Food / Taylor & Francis CRC Press :20~21
Adsul M / 2007 / Production of lactic acid from cellobiose and cellotriose by Lactobacillus delbrueckii mutant Uc-3 / Appl. Environ. Microbiol 73:5055~5057
Atkinson AJ / 2007 / Principles of Clinical Pharmacology / Elsevier Inc :18~20
Balejko E / 2012 / Influence of Lactobacillus rhamnosus gg methabolites on growth of periodontal diseases bacteria / Electron. J. Polish Agric. Univ 15:1~7
Berdanier CD / 2007 / Handbook of Nutrition and Food / Taylor & Francis CRC Press :20~21
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